ZFPM1 Polyclonal antibody, PBS Only

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• ZFPM1 Polyclonal antibody, PBS Only (28012-1-PBS)

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  WB analysis of mouse liver using 28012-1-AP (same clone as 28012-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 28012-1-AP (ZFPM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28012-1-PBS in a different storage buffer formulation.

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  WB analysis using 28012-1-AP (same clone as 28012-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28012-1-AP (ZFPM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28012-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 28012-1-AP (same clone as 28012-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28012-1-AP (ZFPM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28012-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 28012-1-AP (same clone as 28012-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28012-1-AP (ZFPM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28012-1-PBS in a different storage buffer formulation.

ZFPM1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 28012-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

28012-1-PBS targets ZFPM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27829

  Product name: Recombinant human ZFPM1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 331-506 aa of NM_153813

  Sequence: MTKANCERHLKVHTDTLSGVCHSCGFISTTRDILYSHLVTNHMVCQPGSKGEIYSPGAGHPATKLPPDSLGSFQQQHTALQGPLASADLGLAPTPSPGLDRKALAEATNGEARAEPLAQNGGSSEPPAAPRSIKVEAVEEPEAAPILGPGEPGPQAPSRTPSPRSPAPARVKAELSS

• Full Name: zinc finger protein, multitype 1
• Calculated Molecular Weight: 105 aa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: NM_153813
• Gene Symbol: ZFPM1
• Gene ID (NCBI): 161882
• RRID: AB_3086021
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IX07
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ZFPM1 (Zinc Finger Protein, FOG Family Member 1, previous known as FOG1) is a protein with nine zinc finger domains, the C2HC type zinc fingers on ZFPM1 protein are responsible for mediating the interaction with the N-finger of GATA factors (PMID:10329627). ZFPM1 also contains two domains that are known to recruit transcriptional regulatory complexes. The one located at the extreme N-terminus is essential to recruit the nucleosome remodeling and histone deacetylase (NuRD) complex, another one between zinc fingers 6 and 7 could bind to CtBP co-repressor (PMID:30611731).