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ZFYVE16 Polyclonal antibody, PBS Only (13118-2-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis using 13118-2-AP (same clone as 13118-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.
WB analysis of mouse kidney using 13118-2-AP (same clone as 13118-2-PBS)
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.
WB analysis of human placenta using 13118-2-AP (same clone as 13118-2-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 13118-2-AP (same clone as 13118-2-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.
WB analysis of HeLa using 13118-2-AP (same clone as 13118-2-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 13118-2-AP (ZFYVE16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.
IP experiment of HeLa using 13118-2-AP (same clone as 13118-2-PBS)
IP result of anti-ZFYVE16 (IP:13118-2-AP, 5ug; Detection:13118-2-AP 1:500) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.
IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.
IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.
IHC staining of human heart using 13118-2-AP (same clone as 13118-2-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.
IHC staining of human heart using 13118-2-AP (same clone as 13118-2-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.
IHC staining of human placenta using 13118-2-AP (same clone as 13118-2-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.
IHC staining of human placenta using 13118-2-AP (same clone as 13118-2-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.
IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.
IHC staining of human kidney using 13118-2-AP (same clone as 13118-2-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 13118-2-AP (ZFYVE16 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.
IF Staining of HeLa using 13118-2-AP (same clone as 13118-2-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZFYVE16 antibody (13118-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). CL594-Phalloidin (red). DAPI (blue) This data was developed using the same antibody clone with 13118-2-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

ZFYVE16 Polyclonal antibody, PBS Only

Name: ZFYVE16 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 13118-2-PBS

ZFYVE16 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 13118-2-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

13118-2-PBS targets ZFYVE16 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3811 Product name: Recombinant human ZFYVE16 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-306 aa of BC032227 Sequence: MDSYFKAAVSDLDKLLDDFEQNPDEQDYLQDVQNAYDSNHCSVSSELASSQRTSLLPKDQECVNSCASSETSYGTNESSLNEKTLKGLTSIQNEKNVTGLDLLSSVDGGTSDEIQPLYMGRCSKPICDLISDMGNLVHATNSEEDIKKLLPDDFKSNADSLIGLDLSSVSDTPCVSSTDHDSDTVREQQNDTSSELQNREIGGIKELGIKVDTTLSDSYNYSGTENLKDKKIFNQLESIVDFNMSSALTRQSSKMFHAKDKLQHKSQPCGLLKDVGLVKEEVDVAVITAAECLKEEGKTSALTCSL Predict reactive species
Full Name	zinc finger, FYVE domain containing 16
Calculated Molecular Weight	1539 aa, 169 kDa
Observed Molecular Weight	230-250 kDa
GenBank Accession Number	BC032227
Gene Symbol	ZFYVE16
Gene ID (NCBI)	9765
RRID	AB_2288795
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q7Z3T8
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Endofin (also known as ZFYVE16) is an endosomal FYVE domain protein implicated in regulating membrane trafficking. Endofin has been shown to localize to early endosomes. It co-localizes with SARA but does not associate with it or Smad2 and does not behave like SARA in affecting TGF-signaling. Northern blot analysis showed ZFYVE16 was widely expressed with high levels in kidney, placenta and lung. Overexpression of Endofin causes endosome aggregation. The gene of ZFYVE16 maps to chromosome 5q14, and encodes a 1539-amino acid protein with a molecular mass of 169 kDa. In addition, endogenous Endofin can also be detected as a band of 230-250 kDa. (PMID: 11546807)