ZFYVE28 Polyclonal antibody

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  WB analysis using 25360-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25360-1-AP (ZFYVE28 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 25360-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZFYVE28 antibody (25360-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 25360-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZFYVE28 antibody (25360-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

ZFYVE28 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 25360-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Zinc finger FYVE domain-containing protein 28, LST2, KIAA1643, hLst2

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25360-1-AP targets ZFYVE28 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19796

  Product name: Recombinant human ZFYVE28 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 575-887 aa of BC137309

  Sequence: EDVVERLREKCSPGGVIGASYAAGLAKASDRAPERQEEAPPPSEDASNGREPKAPTSDKCLPHTSGSQVDTASGLQGEAGVAGQQEPEARELHAGSPPAHEAPQALSGSSSSTAGSCSSDKMGPEAAPAATHAAPQATREKIRSRFHGSHDLIHRLFVCISGVADQLQTNYASDLRSILKTLFEVMATKPETDDKEKLRKVTQTLRSAALEDCALCQETLSSSELAAKTRDGDFEDPPEWVPDEACGFCTACKAPFTVIRRKHHCRSCGKIFCSRCSSHSAPLPRYGQVKPVRVCTHCYMFHVTPFYSDKAGL

• Full Name: zinc finger, FYVE domain containing 28
• Calculated Molecular Weight: 887 aa, 96 kDa
• Observed Molecular Weight: 96 kDa
• GenBank Accession Number: BC137309
• Gene Symbol: ZFYVE28
• Gene ID (NCBI): 57732
• RRID: AB_3085790
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HCC9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZFYVE28, also known as LST2, belongs to the lst-2 family. ZFYVE28 is a FYVE domain protein that has been shown to be a regulator of the epidermal growth factor receptor (EGFR) pathway. The non-ubiquitinylated form of ZFYVE28 co-localizes with EGFR in endosomes and promotes receptor degradation (PMID: 29549365). ZFYVE28 has 8 isoforms with the molecular mass of 19-33, 83-93, and 96 kDa.