SARA Polyclonal antibody

• Featured Product
• KD/KO Validated

SARA Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14821-1-AP

Synonyms

hSARA, Madh interacting protein, MADHIP, Novel serine protease, NSP, Receptor activation anchor, SARA, SMADIP, ZFYVE9

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Validation Data Gallery

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  WB analysis of mouse brain using 14821-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14821-1-AP (SARA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Product Information

14821-1-AP targets SARA in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SARA fusion protein Ag6592
• Full Name: zinc finger, FYVE domain containing 9
• Calculated Molecular Weight: 1425 aa, 156 kDa
• GenBank Accession Number: BC032680
• Gene Symbol: ZFYVE9
• Gene ID (NCBI): 9372
• RRID: AB_2219204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZFYVE9, also known as SARA (Smad anchor for receptor activation), is a double zinc finger (FYVE domain) protein that interacts directly with SMAD2 and SMAD3, and is involved in Alzheimer's disease. SARA functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. SARA plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. SARA is possibly associated with TGF-beta receptor internalization.

Publications

Species	Application	Title
mouse	IF	Mol Psychiatry; WDR81 regulates adult hippocampal neurogenesis through endosomal SARA-TGFβ signaling.; Authors - Min Wang ; KD Validated
human	WB	Oncotarget; TGFBR-IDH1-Cav1 axis promotes TGF-β signalling in cancer-associated fibroblast.; Authors - Xiaodan Hou
human	WB, IF	J Biol Chem; Phosphatidylinositol 3-kinase and Rab5 GTPase inversely regulate the Smad anchor for receptor activation (SARA) protein independently of transforming growth factor-β1.; Authors - Runyan Constance E CE
mouse	WB	Stem Cell Reports; Dachshund Depletion Disrupts Mammary Gland Development and Diverts the Composition of the Mammary Gland Progenitor Pool.; Authors - Xuanmao Jiao