ZKSCAN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ZKSCAN1 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF

Conjugate

Unconjugated

Cat no : 25214-1-AP

Synonyms

9130423L19Rik, KOX18, PHZ 37, Zinc finger protein 139, Zinc finger protein 36, Zinc finger protein KOX18, ZKSCAN1, ZNF139, ZNF36

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Validation Data Gallery

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  WB analysis using 25214-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25214-1-AP (ZKSCAN1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25214-1-AP

  WB result of ZKSCAN1 antibody (25214-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZKSCAN1 transfected HEK-293 cells.

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  IF Staining of A431 using 25214-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ZKSCAN1 antibody (25214-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, MCF-7 cells
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25214-1-AP is ZKSCAN1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZKSCAN1 fusion protein Ag19721
• Full Name: zinc finger with KRAB and SCAN domains 1
• Calculated Molecular Weight: 563 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC022378
• Gene Symbol: ZKSCAN1
• Gene ID (NCBI): 7586
• RRID: AB_2879963
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ZKSCAN1(Zinc finger protein with KRAB and SCAN domains 1), also named as ZNF139, is a member in transcription factor zinc finger protein family(PMID: 24515389). Zinc finger protein 139 (ZNF139) gene is proved play an important role in gastric cancer, overexpression of ZNF139 correlated with tumor differentiation, invasion depth, clinical stage, lymphatic metastasis, and blood vessel invasion(PMID: 25436386, PMID: 25561801). The molecular weight of ZKSCAN1 is 64 kDa.