ZP2 Polyclonal antibody, PBS Only

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• ZP2 Polyclonal antibody, PBS Only (21832-1-PBS)

• 

  WB analysis using 21832-1-AP (same clone as 21832-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21832-1-AP (ZP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21832-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse ovary using 21832-1-AP (same clone as 21832-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse ovary tissue using ZP2 antibody (21832-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21832-1-PBS in a different storage buffer formulation.

ZP2 Polyclonal Antibody for WB, IF-P, Indirect ELISA

Cat No. 21832-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, Indirect ELISA

Synonyms

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Product Information

21832-1-PBS targets ZP2 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16386

  Product name: Recombinant human ZP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 440-745 aa of BC096304

  Sequence: EIHALWTDFPPSKISRDSEFRMTVKCSYSRNDMLLNINVESLTPPVASVKLGPFTLILQSYPDNSYQQPYGENEYPLVRFLRQPIYMEVRVLNRDDPNIKLVLDDCWATSTMDPDSFPQWNVVVDGCAYDLDNYQTTFHPVGSSVTHPDHYQRFDMKAFAFVSEAHVLSSLVYFHCSALICNRLSPDSPLCSVTCPVSSRHRRATGATEAEKMTVSLPGPILLLSDDSSFRGVGSSDLKASGSSGEKSRSETGEEVGSRGAMDTKGHKTAGDVGSKAVAAVAAFAGVVATLGFIYYLYEKRTVSNH

• Full Name: zona pellucida glycoprotein 2 (sperm receptor)
• Calculated Molecular Weight: 745 aa, 82 kDa
• Observed Molecular Weight: 75-82 kDa
• GenBank Accession Number: BC096304
• Gene Symbol: ZP2
• Gene ID (NCBI): 7783
• RRID: AB_3669375
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q05996
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ZP2 is a major component of the zona pellucida to which sperm bind prior to penetrating the zona matrix and fusing with the egg. Following fertilization and cortical granule exocytosis, ZP2 is cleaved by ovastacin after which sperm do not bind to the zona pellucida. The zona pellucida and ovastacin proteins transverse the endomembrane system during oogenesis during which passage ZP2 remains intact. Secreted ZP2 is incorporated into the extracellular zona pellucida surrounding ovulated eggs and is cleaved in the zona matrix surrounding 2-cell embryos. (PMID: 28114310)