ZSCAN5A Polyclonal antibody

ZSCAN5A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19926-1-AP

Synonyms

Zinc finger protein 495, ZNF495, ZSCAN5, ZSCAN5A

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Validation Data Gallery

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  WB analysis using 19926-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19926-1-AP (ZSCAN5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 19926-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19926-1-AP (ZSCAN5A antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 19926-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19926-1-AP (ZSCAN5A antibody) at dilution of 1:200 (under 10x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19926-1-AP targets ZSCAN5A in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZSCAN5A fusion protein Ag13898
• Full Name: zinc finger and SCAN domain containing 5A
• Calculated Molecular Weight: 496 aa, 56 kDa
• GenBank Accession Number: BC002636
• Gene Symbol: ZSCAN5A
• Gene ID (NCBI): 79149
• RRID: AB_2878624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zinc finger and SCAN domain-containing protein 5A (ZSCAN5A), also known as ZNF495 or ZSCAN5, belongs to the ZSCAN family of transcription factors. ZSCAN transcription factors contain two major domains, the zinc finger domain and the SCAN box. ZSCAN5A has two isoforms and is predicted to be located in the nucleus, it is also predicted to be involved in the regulation of transcription by RNA polymerase II and may be involved in angiogenesis, cell apoptosis, differentiation, migration and invasion, proliferation (PMID: 31218096).