CoraLite® Plus 647-conjugated a-SMA specific Recombinant monoclonal antibody

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• CoraLite® Plus 647-conjugated a-SMA specific Recombinant monoclonal antibody (CL647-80008-2)

a-SMA specific Uni-rAb^® Recombinant Antibody for IF-P

Cat No. CL647-80008-2

Clone No.250297G8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P

Synonyms

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, a SMA

• 

  IF Staining of mouse heart using CL647-80008-2

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite® Plus 647 a-SMA specific antibody (CL647-80008-2, Clone: 250297G8 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-80008-2 targets a-SMA specific in IF-P applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: actin, alpha 2, smooth muscle, aorta
• Calculated Molecular Weight: 42 kDa
• GenBank Accession Number: NM_001613
• Gene Symbol: Alpha smooth muscle actin
• Gene ID (NCBI): 59
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P62736
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Alpha smooth muscle actin (α-SMA or ACTA2) is an actin isoform predominantly expressed in smooth muscle cells and is the most widely recognized marker for activated myofibroblasts. In healthy adult tissues, its expression is largely restricted to vascular and visceral smooth muscle. However, during tissue injury, fibrosis, or wound healing, fibroblasts differentiate into myofibroblasts with significant upregulation of α-SMA. Therefore, α-SMA serves as a critical immunohistochemical marker for detecting myofibroblast activation and studying pathological processes such as organ fibrosis (e.g., liver, kidney, lung) and cancer stroma remodeling.