ChromoTek anti-CD4 recombinant VHH, for 2x Cys conjugation

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• anti-CD4 recombinant VHH, for 2x Cys conjugation (MG-1229CC)

Validation Data Gallery

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  Affinity Data
  BLI analysis of the interaction between varying concentrations of anti-CD4 recombinant VHH (MG-1229CC, Clone: CTK0242) and immobilized, biotinylated CD4 protein.

The small size of VHHs (~15 kDa) enables access to epitopes that may be sterically inaccessible to full size IgGs. Their reduced steric hindrance also facilitates simultaneous staining of multiple targets, making them particularly useful for applications like Nano-Flow. MG-1229CC is an unconjugated recombinant anti-CD4 Nanobody (VHH). Suitable for for dual cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteines reduced.

Cat No. MG-1229CC

Host/Type

Alpaca VHH

Reactivity

Human

Applications

Conjugation

Conjugate

Unconjugated

Synonyms

T4 VHH, CD4 VHH

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Product Information

The small size of VHHs (~15 kDa) enables access to epitopes that may be sterically inaccessible to full size IgGs. Their reduced steric hindrance also facilitates simultaneous staining of multiple targets, making them particularly useful for applications like Nano-Flow. MG-1229CC is an unconjugated recombinant anti-CD4 Nanobody (VHH). Suitable for for dual cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteines reduced.

• Applications: Conjugation
• Reactivity: Human
• Conjugate: Unconjugated
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity: 17 nM
• Clone: CTK0242
• Immunogen: 26-396 aa
• Molecular Weight: 14.8 kDa
• Form: Liquid
• RRID: /
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 0.09% sodium azide, 1mM TCEP
• Storage Condition: Store at -20°C
• Shipping: dry ice
• Sizes: 250 µl / 500 µg
• Background: CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

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