ChromoTek anti-Mouse IgG2a VHH Agarose for Immunoprecipitation

Validation Data Gallery

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  IP of Alpha-2-Macroglobulin (A2M) by anti-mouse IgG2a VHH agarose (mIG2aa) using the A2M monoclonal antibody 4B11F7 (Proteintech: 66126-1-Ig). As control, a IgG2a isotype control antibody (Proteintech: 66360-2-Ig) was used (BISO). 5 µg of each IgG was spiked into human serum which was diluted 1:10. 0.3% of input (IN) and 12.5% of bound (B) fraction was loaded onto an SDS-PAGE gel. For Western blot analysis A2M was detected using a polyclonal rabbit IgG (Proteintech: 13545-1-AP) (1:5000) labeled with FlexAble HRP (Proteintech: KFA045). Compared to protein A and G agarose, mIG2aa shows a superior precipitation and a cleaner detection of A2M.

anti-Mouse IgG2a IP Beads is an affinity resin for IP of Mouse IgG2a. It consists of rabbit specific VHHs (Nanobodies) coupled to Agarose beads.

Cat No. mIG2aa

Reactivity

Mouse IgG2a

Applications

IP, Co-IP

Host/Type

Alpaca, recombinant VHH

Conjugate

Agarose

Synonyms

Mouse IP, IgG IP, IgG Trap, Secondary VHH, VHH, Nanobody

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Product Information

anti-Mouse IgG2a IP Beads is an affinity resin for IP of Mouse IgG2a. It consists of rabbit specific VHHs (Nanobodies) coupled to Agarose beads.

• Description: Ready to use VHH coupled Agarose Beads for Immunoprecipitation of Mouse IgG2a. Add bulletpoints from below here:;

  • Fast, reliable & efficient one-step immunoprecipitation;

  • Ready-to-use;

  • Stable in harsh buffer conditions;

  • Suitable for downstream mass spec analysis;

  • High Specificity;

  • Low background

• Applications: IP, Co-IP
• Binding capacity: /
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: SDS Sample Buffer
• Wash buffer compatibility: /
• Type: Nanobody
• Class: Recombinant - Animal free production
• Host: Alpaca
• Affinity (KD): /
• Immunogen: Mouse IgG2a
• RRID: AB_3697094
• Storage Buffer: 20% Ethanol
• Storage Condition: +4°C / do not freeze!
• Shipping: Ambient Temp
• Size: 1 ml; 2 ml; 5 ml

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