ChromoTek anti-Mouse IgG3 Magnetic VHH Agarose for Immunoprecipitation

Validation Data Gallery

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  IP of GRP75 by anti-mouse IgG3 magnetic VHH agarose (mIG3ma) using an anti-GRP75 monoclonal IgG3. As control, a IgG3 isotype control antibody (PTG: 66360-4-Ig) was used (BISO). 5 µg of each IgG was spiked into HEK293T cell lysate derived from 1x10^7 cells. 0.5% of input (IN) and flow through (FT) and 15% of bound (B) fraction was loaded onto an SDS-PAGE gel. For Western blot analysis GRP75 was detected using a polyclonal rabbit IgG (PTG: 14887-1-AP) (1:5000) labeled with FlexAble HRP (KFA005).

anti-Mouse IgG3 IP Beads is an affinity resin for IP of Mouse IgG3. It consists of rabbit specific VHHs (Nanobodies) coupled to Magnetic Agarose beads.

Cat No. mIG3ma

Reactivity

Mouse IgG3

Applications

IP, Co-IP

Host/Type

Alpaca, recombinant VHH

Conjugate

Magnetic Agarose

Synonyms

Mouse IP, IgG IP, IgG Trap, Secondary VHH, VHH, Nanobody

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Product Information

anti-Mouse IgG3 IP Beads is an affinity resin for IP of Mouse IgG3. It consists of rabbit specific VHHs (Nanobodies) coupled to Magnetic Agarose beads.

• Description: Ready to use VHH coupled Magnetic Agarose Beads for Immunoprecipitation of Mouse IgG3.;

  • Fast, reliable and efficient one-step Immunoprecipitation;

  • Ready-to-use;

  • Stable in harsh buffer conditions;

  • Suitable for downstream mass spec analysis;

  • High Specificity;

  • Low background

• Applications: IP, Co-IP
• Conjugate: Magnetic Agarose beads; ~40 um (cross-linked 6% magnetic agarose beads)
• Elution buffer: SDS Sample Buffer
• Type: Nanobody
• Class: Recombinant - Animal free production
• Host: Alpaca
• Immunogen: Mouse IgG3
• RRID: /
• Storage Buffer: 20% Ethanol
• Storage Condition: +4°C / do not freeze!
• Shipping: Ambient Temp
• Size: 1 ml; 2 ml; 5 ml

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