Beta Catenin Polyclonal antibody

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  WB analysis using 17565-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17565-1-AP (Beta Catenin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 17565-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17565-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of mouse brain using 17565-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of HEK-293 using 17565-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17565-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of MCF-7 using 17565-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of mouse liver using 17565-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 17565-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 17565-1-AP

  IP result of anti-Beta Catenin (IP:17565-1-AP, 4ug; Detection:17565-1-AP 1:5000) with HEK-293 cells lysate 1920 ug.

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  IHC staining of human liver cancer using 17565-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17565-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17565-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17565-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 17565-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, green).

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  IF Staining of U-251 using 17565-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of MCF-7 using 17565-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, green).

• 

  IF Staining of T-47D using 17565-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  IF Staining of MCF-7 using 17565-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 17565-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Beta Catenin (17565-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

Beta Catenin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 17565-1-AP

Publications(121)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

CTNNB, Catenin beta-1, Catenin beta 1, beta-Catenin, B-catenin

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells, HeLa cells, mouse brain tissue, A431 cells, MCF-7 cells, mouse liver tissue, rat brain tissue, C6 cells, rat liver tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells, T-47D cells, U-251 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 98 publications below
• IHC: See 19 publications below
• IF: See 32 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Product Information

17565-1-AP targets Beta Catenin in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Beta Catenin fusion protein Ag11763
• Full Name: catenin (cadherin-associated protein), beta 1, 88kDa
• Calculated Molecular Weight: 781 aa, 86 kDa
• Observed Molecular Weight: 92 kDa
• GenBank Accession Number: BC058926
• Gene Symbol: Beta Catenin
• Gene ID (NCBI): 1499
• ENSEMBL Gene ID: ENSG00000168036
• RRID: AB_2088102
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35222
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Publications

Species	Application	Title
mouse	WB,IF	J Clin Invest; JAM3 maintains leukemia-initiating cell self-renewal through LRP5/AKT/β-catenin/CCND1 signaling.; Authors - Yaping Zhang
human	WB,IHC,IF,IP	Cell Death Dis; USP8 positively regulates hepatocellular carcinoma tumorigenesis and confers ferroptosis resistance through β-catenin stabilization; Authors - Jianing Tang
rat	WB,IF	Mater Today Bio; A novel adhesive dual-sensitive hydrogel for sustained release of exosomes derived from M2 macrophages promotes repair of bone defects; Authors - Xiaoying Zhao
human	IHC,IF	Br J Pharmacol; Reregulation of hepatic stellate cell contraction and cirrhotic portal hypertension by Wnt/β-catenin signaling via interaction with Gli1.; Authors - Feng Zhang
human	WB	Acta Pharmacol Sin; Identification of HMGCR as the anticancer target of physapubenolide against melanoma cells by in silico target prediction.; Authors - Hai-Yan Wang
human		Int J Nanomedicine; Self-Assembly Of Retinoid Nanoparticles For Melanoma Therapy.; Authors - Han Liao