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- KD/KO Validated
Beta Catenin Polyclonal antibody
Beta Catenin Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, CoIP, ELISA
Cat no : 17565-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, NIH/3T3 cells, HeLa cells, mouse brain tissue, A431 cells, MCF-7 cells, mouse liver tissue, rat brain tissue, C6 cells, rat liver tissue|
|Positive IHC detected in||human liver cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, T-47D cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 76 publications below|
|IHC||See 15 publications below|
|IF||See 25 publications below|
|IP||See 2 publications below|
|CoIP||See 1 publications below|
17565-1-AP targets Beta Catenin in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Beta Catenin fusion protein Ag11763|
|Full Name||catenin (cadherin-associated protein), beta 1, 88kDa|
|Calculated molecular weight||781 aa, 86 kDa|
|Observed molecular weight||92 kDa|
|GenBank accession number||BC058926|
|Gene ID (NCBI)||1499|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
|Product Specific Protocols|
|WB protocol for Beta Catenin antibody 17565-1-AP||Download protocol|
|IHC protocol for Beta Catenin antibody 17565-1-AP||Download protocol|
|IF protocol for Beta Catenin antibody 17565-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Br J Pharmacol
Reregulation of hepatic stellate cell contraction and cirrhotic portal hypertension by Wnt/β-catenin signaling via interaction with Gli1.
Loss of chemerin triggers bone remodeling in vivo and in vitro.
J Med Chem
Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.
Cell Death Dis
Wnt signaling induces radioresistance through upregulating HMGB1 in esophageal squamous cell carcinoma.
Cell Death Dis
Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation.
Aging (Albany NY)
The Interleukin-33/ST2 axis promotes glioma mesenchymal transition, stemness and TMZ resistance via JNK activation.