- Featured Product
- KD/KO Validated
Beta Catenin Polyclonal antibody
Beta Catenin Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Cat no : 17565-1-AP
Validation Data Gallery
|Positive WB detected in
|HEK-293 cells, NIH/3T3 cells, HeLa cells, mouse brain tissue, A431 cells, MCF-7 cells, mouse liver tissue, rat brain tissue, C6 cells, rat liver tissue
|Positive IHC detected in
|human liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|MCF-7 cells, T-47D cells
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:16000
|IHC : 1:50-1:500
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.40 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
17565-1-AP targets Beta Catenin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|Beta Catenin fusion protein Ag11763
|catenin (cadherin-associated protein), beta 1, 88kDa
|Calculated molecular weight
|781 aa, 86 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
|Product Specific Protocols
|WB protocol for Beta Catenin antibody 17565-1-AP
|IHC protocol for Beta Catenin antibody 17565-1-AP
|IF protocol for Beta Catenin antibody 17565-1-AP
|FC protocol for Beta Catenin antibody 17565-1-AP
|Click here to view our Standard Protocols
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USP8 positively regulates hepatocellular carcinoma tumorigenesis and confers ferroptosis resistance through β-catenin stabilization
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Reregulation of hepatic stellate cell contraction and cirrhotic portal hypertension by Wnt/β-catenin signaling via interaction with Gli1.
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Identification of HMGCR as the anticancer target of physapubenolide against melanoma cells by in silico target prediction.
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Self-Assembly Of Retinoid Nanoparticles For Melanoma Therapy.