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Beta Catenin Polyclonal antibody
Beta Catenin Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 17565-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, NIH/3T3 cells, HeLa cells, mouse brain tissue, A431 cells, MCF-7 cells, mouse liver tissue, rat brain tissue, C6 cells, rat liver tissue |
Positive IHC detected in | human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells, T-47D cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 76 publications below |
IHC | See 15 publications below |
IF | See 25 publications below |
IP | See 2 publications below |
CoIP | See 1 publications below |
Product Information
17565-1-AP targets Beta Catenin in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Beta Catenin fusion protein Ag11763 |
Full Name | catenin (cadherin-associated protein), beta 1, 88kDa |
Calculated molecular weight | 781 aa, 86 kDa |
Observed molecular weight | 92 kDa |
GenBank accession number | BC058926 |
Gene symbol | CTNNB1 |
Gene ID (NCBI) | 1499 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
Protocols
Product Specific Protocols | |
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WB protocol for Beta Catenin antibody 17565-1-AP | Download protocol |
IHC protocol for Beta Catenin antibody 17565-1-AP | Download protocol |
IF protocol for Beta Catenin antibody 17565-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Br J Pharmacol Reregulation of hepatic stellate cell contraction and cirrhotic portal hypertension by Wnt/β-catenin signaling via interaction with Gli1. | ||
J Med Chem Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma. | ||
Cell Death Dis Wnt signaling induces radioresistance through upregulating HMGB1 in esophageal squamous cell carcinoma. | ||
Cell Death Dis Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation. | ||
Aging (Albany NY) The Interleukin-33/ST2 axis promotes glioma mesenchymal transition, stemness and TMZ resistance via JNK activation. |