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Beta Catenin Polyclonal antibody

Beta Catenin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17565-1-AP

Synonyms

b cat, Beta catenin, beta-Catenin, Catenin beta 1, CTNNB, CTNNB1, PRO2286, β catenin, β-catenin



Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells, HeLa cells, mouse brain tissue, A431 cells, MCF-7 cells, mouse liver tissue, rat brain tissue, C6 cells, rat liver tissue
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, T-47D cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

17565-1-AP targets Beta Catenin in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Beta Catenin fusion protein Ag11763
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Calculated molecular weight 781 aa, 86 kDa
Observed molecular weight 92 kDa
GenBank accession numberBC058926
Gene symbol CTNNB1
Gene ID (NCBI) 1499
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Protocols

Product Specific Protocols
WB protocol for Beta Catenin antibody 17565-1-APDownload protocol
IHC protocol for Beta Catenin antibody 17565-1-APDownload protocol
IF protocol for Beta Catenin antibody 17565-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Br J Pharmacol

Reregulation of hepatic stellate cell contraction and cirrhotic portal hypertension by Wnt/β-catenin signaling via interaction with Gli1.

Authors - Feng Zhang
mouseWB

Mol Metab

Loss of chemerin triggers bone remodeling in vivo and in vitro.

Authors - Long Han
humanWB

J Med Chem

Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.

Authors - Zongyuan Zhou
mouseWB,IF

Cell Death Dis

Wnt signaling induces radioresistance through upregulating HMGB1 in esophageal squamous cell carcinoma.

Authors - Yuanyuan Zhao
WB,IF

Cell Death Dis

Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation.

Authors - Yue Zhang
humanWB

Aging (Albany NY)

The Interleukin-33/ST2 axis promotes glioma mesenchymal transition, stemness and TMZ resistance via JNK activation.

Authors - Lin Lin