beta IG-H3/TGFBI Recombinant monoclonal antibody, PBS Only (Capture)

• 

  Sandwich ELISA sample test of MP02279-2

  The mean TGFBI/BIGH3 concentration was determined to be 136.1 ng/mL in HUVEC cell extract based on a 2.2 mg/mL extract load.

• 

  Sandwich ELISA sample test of MP02279-2

  HUVEC cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for human TGFBI/BIGH3, and measured 7.9 ng/mL.

• 

  Sandwich ELISA sample test of MP02279-2

  Plasma of six individual healthy human donors was measured. The TGFBI/BIGH3 concentration of detected samples was determined to be 1,016.5 ng/mL with a range of 640.9-1,282.5 ng/mL.

• 

  Sandwich ELISA standard curve of MP02279-2

  Sandwich ELISA standard curve of MP02279-2, Human TGFBI/BIGH3 Recombinant Matched Antibody Pair - PBS only. 80805-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1233. 80805-2-PBS was HRP conjugated as the detection antibody. Range: 97.7-6250 pg/mL.

• 

  Affinity and Kinetic Characterization of 80805-5-PBS

  Biolayer interferometry (BLl) kinetic assays of 80805-5-PBS against Human beta IG-H3/TGFBI were performed. The affinity constant is below 1 pM.

beta IG-H3/TGFBI Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Cat No. 80805-5-PBS

Clone No.243108F11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA

Synonyms

TGFBI, TGFBI / BIGH3, Beta ig h3, Beta ig-h3, BIGH3

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Product Information

80805-5-PBS targets beta IG-H3/TGFBI as part of a matched antibody pair:

MP02279-2: 80805-5-PBS capture and 80805-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1233

  Product name: recombinant human beta IG-H3/TGFBI protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 24-683 aa of NM_000358.3

  Sequence: GPAKSPYQLVLQHSRLRGRQHGPNVCAVQKVIGTNRKYFTNCKQWYQRKICGKSTVISYECCPGYEKVPGEKGCPAALPLSNLYETLGVVGSTTTQLYTDRTEKLRPEMEGPGSFTIFAPSNEAWASLPAEVLDSLVSNVNIELLNALRYHMVGRRVLTDELKHGMTLTSMYQNSNIQIHHYPNGIVTVNCARLLKADHHATNGVVHLIDKVISTITNNIQQIIEIEDTFETLRAAVAASGLNTMLEGNGQYTLLAPTNEAFEKIPSETLNRILGDPEALRDLLNNHILKSAMCAEAIVAGLSVETLEGTTLEVGCSGDMLTINGKAIISNKDILATNGVIHYIDELLIPDSAKTLFELAAESDVSTAIDLFRQAGLGNHLSGSERLTLLAPLNSVFKDGTPPIDAHTRNLLRNHIIKDQLASKYLYHGQTLETLGGKKLRVFVYRNSLCIENSCIAAHDKRGRYGTLFTMDRVLTPPMGTVMDVLKGDNRFSMLVAAIQSAGLTETLNREGVYTVFAPTNEAFRALPPRERSRLLGDAKELANILKYHIGDEILVSGGIGALVRLKSLQGDKLEVSLKNNVVSVNKEPVAEPDIMATNGVVHVITNVLQPPANRPQERGDELADSALEIFKQASAFSRASQRSVRLAPVYQKLLERMKH

• Full Name: transforming growth factor, beta-induced, 68kDa
• Calculated Molecular Weight: 75kDa
• GenBank Accession Number: NM_000358.3
• Gene Symbol: TGFBI
• Gene ID (NCBI): 7045
• ENSEMBL Gene ID: ENSG00000120708
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15582
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.