|Positive WB detected in||HeLa cells, RAW 264.7 cells, CHO cells, HepG2 cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, 4T1 cells, PC-12 cells, HSC-T6 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
60315-1-Ig targets c-SRC in WB, IHC, IF, ELISA applications and shows reactivity with human, hamster, mouse, rat samples.
|Tested Reactivity||human, hamster, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||c-SRC fusion protein Ag22306|
|Full Name||v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)|
|Calculated molecular weight||60 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||BC011566|
|Gene ID (NCBI)||6714|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase.
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