ChromoTek Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102]
Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH is an anti-human IgG and anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 2 Nanobodies that bind to human IgG and rabbit IgG with high affinity & specificity.
Reactivity
Rabbit, Human, Macaque
Applications
IF, WB, FC
Conjugate
Alexa Fluor® 488
Cat no : srbAF488-1
Synonyms
Validation Data Gallery
Product Information
Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH is an anti-human IgG and anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 2 Nanobodies that bind to human IgG and rabbit IgG with high affinity & specificity.
Applications | IF, WB, FC |
Recommended Dilution | Immunofluorescence 1:1,000 Super-resolution microscopy 1:1,000 Western blot 1:1,000 Optimal working concentration is application-dependent and should be determined by testing a dilution range from 1:250 to 1:2,000. Note: Image acquisition time may have to be optimized |
Clonality | Biclonal: mixture of 2 monoclonal Nanobodies |
Host | Alpaca |
Affinity | CTK0101: KD = 0.2 nM, CTK0102: KD = 1.2 nM |
Specificity/Target | Human IgG and rabbit IgG |
Reactivity | Rabbit, Human, Macaque |
Conjugate | Alexa Fluor® 488 |
Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide |
Storage Condition | Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles. |
Documentation
SDS |
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SDS Nano-Secondary® alpaca anti-human IgG, anti-rabbit IgG, Alexa Fluor® 488 (PDF) |
Protocol |
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Protocol Nano-Secondary® alpaca anti-human IgG, anti-rabbit IgG, Alexa Fluor® 488 (PDF) |
Application note |
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One-step incubation with Nano-Secondaries (PDF) |
Blocking recommendation for immunostaining with Nano-Secondaries (PDF) |
Brochure |
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Chromotek nanobodies brochure (PDF) |
Publications
Application | Title |
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Cell Physiol Biochem Studies on the Role of the Transcription Factor Tcf21 in the Transdifferentiation of Parietal Epithelial Cells into Podocyte-Like Cells. | |
J Cell Mol Med ScoMorphoFISH: A deep learning enabled toolbox for single-cell single-mRNA quantification and correlative (ultra-)morphometry. | |
Anal Chem Noncompetitive Fluorescence Polarization Immunoassay for Protein Determination. | |
Basic Res Cardiol Inhibition of macrophage proliferation dominates plaque regression in response to cholesterol lowering. | |
Nat Commun Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer. | |
Sci Adv Mutation of SLC7A14 causes auditory neuropathy and retinitis pigmentosa mediated by lysosomal dysfunction. |