ChromoTek Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, Alexa Fluor® 568 [CTK0105, CTK0106]

Nano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity.

Reactivity

Mouse

Applications

IF, WB

Conjugate

Alexa Fluor® 568

Publications

1

Cat no : sms2bAF568-1

Synonyms

mouse IgG2b; mouse immunoglobulin Gamma 2b

---

Validation Data Gallery

• 

  Anti-mouse IgG2b Nano-Secondary: Well-defined and characterized immunostaining. Primary anti-mouse IgG2b antibody (grey) with 2X2 monoclonal mouse Fc- specific Nanobodies (green) bound. In total, 8 fluorophores (red stars) label the mouse IgG2b primary antibody.

• 

  The anti-mouse IgG2b Nano-Secondary is subclass-specific and does not cross-react with IgGs from other commonly used species (here rabbit) and with mouse IgG1 and IgG3 subclasses.

• 

  HeLa cells were immunostained with mouse IgG2b anti-βTubulin antibody + alpaca anti-mouse IgG2b VHH Alexa Fluor? 568 (red). Nuclei were stained with DAPI (blue). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich

• 

  One-step immunostaining is the simultaneous incubation of mouse IgG2b primary antibody and anti-mouse IgG2b Nano-Secondary. This method reduces incubation and hands-on time. Simultaneous incubation also supports multiplexing, tissue penetration, and cell staining for flow cytometry.

• 

  Dissociation constant Kd and affinity.

---

Product Information

Nano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity.

• Applications: IF, WB
• Recommended Dilution: Immunofluorescence 1:1,000; Western blot 1:1,000; Optimal working concentration is application-dependent and should be determined by testing a dilution range from 1:250 to 1:2,000.; Note: Image acquisition time may have to be optimized.
• Clonality: VHH
• Host: Alpaca
• Affinity: CTK0105: KD = 5 nM, CTK0106: KD = 0.2 nM
• Specificity/Target: Mouse IgG2b; this Nanobody mixture recognizes the Fc fragment of mouse IgG2b
• Reactivity: Mouse
• Conjugate: Alexa Fluor® 568
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
• Storage Condition: Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles.

Publications

Application	Title
IF	Science; Mechanism of spindle pole organization and instability in human oocytes.; Authors - Chun So