ChromoTek Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, Alexa Fluor® 568 [CTK0105, CTK0106]
Nano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity.
Cat no : sms2bAF568-1
Synonyms
Validation Data Gallery
Product Information
Nano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity.
Applications | IF, WB |
Recommended Dilution | Immunofluorescence 1:1,000 Western blot 1:1,000 Optimal working concentration is application-dependent and should be determined by testing a dilution range from 1:250 to 1:2,000. Note: Image acquisition time may have to be optimized. |
Clonality | VHH |
Host | Alpaca |
Affinity | CTK0105: KD = 5 nM, CTK0106: KD = 0.2 nM |
Specificity/Target | Mouse IgG2b; this Nanobody mixture recognizes the Fc fragment of mouse IgG2b |
Reactivity | Mouse |
Conjugate | Alexa Fluor® 568 |
Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide |
Storage Condition | Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles. |
Documentation
SDS |
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SDS Alpaca anti-mouse IgG2b Alexa Fluor 568 (PDF) |
Protocols |
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Protocol Alpaca anti-mouse IgG2b, Fc-specific recombinant VHH, Alexa Fluor 568 (PDF) |
Application note |
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One-step Incubation with Nano-Secondaries (PDF) |
Blocking recommendation for immunostaining with Nano-Secondaries (PDF) |
Brochure |
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Chromotek nanobodies brochure (PDF) |
Publications
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