ChromoTek Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, Alexa Fluor® 568 [CTK0105, CTK0106]

Validation Data Gallery

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  Anti-mouse IgG2b Nano-Secondary: Well-defined and characterized immunostaining. Primary anti-mouse IgG2b antibody (grey) with 2X2 monoclonal mouse Fc- specific Nanobodies (green) bound. In total, 8 fluorophores (red stars) label the mouse IgG2b primary antibody.

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  HeLa cells were immunostained with mouse IgG2b anti-βTubulin antibody + alpaca anti-mouse IgG2b VHH Alexa Fluor® 568 (red). Nuclei were stained with DAPI (blue). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich

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  One-step immunostaining is the simultaneous incubation of mouse IgG2b primary antibody and anti-mouse IgG2b Nano-Secondary. This method reduces incubation and hands-on time. Simultaneous incubation also supports multiplexing, tissue penetration, and cell staining for flow cytometry.

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  Dissociation constant Kd and affinity.

Nano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity.

Cat No. sms2bAF568-1

Publications(1)

Applications

IF, WB

Conjugate

Alexa Fluor® 568

Host

Alpaca

Reactivity

Mouse IgG2b

Synonyms

mouse IgG2b; mouse immunoglobulin Gamma 2b

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Product Information

Nano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity.

• Description: Nano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity. Nano-Secondary reagents enable simultaneous incubation with primary antibodies, thus shortening your experimental. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below.
• Applications: IF, WB
• Host: Alpaca
• Species Reactivity: Mouse IgG2b Fc-fragment; No cross-reactivity: goat, guinea pig, rabbit, rat, sheep, human, macaque serum proteins, mouse IgG1, IgG2a, IgG2c, IgG3
• Target/Specificity: This Nanobody mixture recognizes the Fc fragment of mouse IgG2b.
• Physical State: Liquid
• Conjugate: Alexa Fluor® 568
• Suggested Dilution: Immunofluorescence 1:1,000; Western blot 1:1,000
• Purification Method: Recombinant expression, affinity purification IMAC
• Type: Mixture of 2 monoclonal Nanobodies
• Class: Recombinant
• Format: Alpaca single domain antibodies, monovalent
• Cross-reactivity: No cross-reactivity to rabbit, rat, sheep, goat, guinea pig, human, and macaque serum
• Immunogen: Purified mouse IgG2b
• Clonality: Biclonal: mixture of 2 monoclonal Nanobodies
• Clones: VHH0275, VHH0288
• RRID: AB_2827582
• Affinity (KD): CTK0105: K_D = 5 nM, CTK0106: K_D = 0.2 nM
• Excitation / Emission: Excitation max: 578 nm, Emission max: 603 nm
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
• Storage Condition: Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles.
• Size: 10 µL; 100 µL
• Note: This product is for research use only, not for diagnostic or therapeutic use

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Publications

Application	Title
IF	Science; Mechanism of spindle pole organization and instability in human oocytes.; Authors - Chun So