ChromoTek Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, Alexa Fluor® 647 [CTK0105, CTK0106]
Nano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity.
Ready to use reagent
Reactivity
Mouse
Applications
IF, WB
Conjugate
Alexa Fluor® 647
Cat no : sms2bAF647-1
Synonyms
Validation Data Gallery
Product Information
Nano-Secondary® alpaca anti-mouse IgG2b, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG2b with high affinity & specificity.
Applications | IF, WB |
Recommended Dilution | Immunofluorescence 1:1,000 Western blot 1:1,000 Optimal working concentration is application-dependent and should be determined by testing a dilution range from 1:250 to 1:2,000. Note: Image acquisition time may have to be optimized. |
Clonality | Biclonal: mixture of 2 monoclonal Nanobodies |
Host | Alpaca |
Affinity | CTK0105: KD = 5 nM, CTK0106: KD = 0.2 nM |
Specificity/Target | Mouse IgG2b; this Nanobody mixture recognizes the Fc fragment of mouse IgG2b |
Reactivity | Mouse |
Conjugate | Alexa Fluor® 647 |
Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide |
Storage Condition | Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles. |
Documentation
SDS |
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SDS Alpaca anti-mouse IgG2b Alexa Fluor 647 (PDF) |
Protocols |
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Protocol Alpaca anti-mouse IgG2b, Fc-specific recombinant VHH, Alexa Fluor 647 (PDF) |
Application note |
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Blocking recommendation for immunostaining with Nano-Secondaries (PDF) |
One-step Incubation with Nano-Secondaries (PDF) |
Brochure |
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Nano-Secondaries product brochure (PDF) |