Nano-Secondary reagents

High resolution and low background

Nano-Secondary® reagents are a novel class of recombinant secondary antibodies designed to provide higher resolution, lower background, and cleaner images. Nano-Secondary reagents have recombinantly generated Nanobodies/ VHHs conjugated to Alexa Fluor® or CoraLite® Plus fluorescent dyes and bind to primary antibodies with high affinity in a species and subtype specific manner:

  • Achieves higher resolution and better tissue penetration due to smaller size
  • Subclass specific enabling multiplexing of same species antibodies
  • Low background
  • High lot-to-lot consistency
  • Reproducible results throughout your project

Nano-Secondary reagents can be applied in:

Explore Nano-Secondary reagents
Staining image of secondary nanobody ChromoTek Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488. The anti-mouse IgG1 Nano-Secondary is subclass-specific and does not cross-react with IgGs from other commonly used species (here rabbit) and with mouse IgG2b and IgG3 subclasses.

Multiplexed immunostaining of HeLa cells with 3 subclass-specific alpaca anti-mouse Nano-Secondary reagents. Grey: Mouse IgG1 anti-Vimentin + alpaca anti-mouse IgG1 VHH Alexa Fluor® 647. Green: Mouse IgG2b anti-Lamin + alpaca anti-mouse IgG2b VHH Alexa Fluor® 488. Red: Mouse IgG3 anti-MOT + alpaca anti-mouse IgG3 VHH Alexa Fluor® 568. Scale bar, 10 µm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.