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Recombinant Human B7-H3/CD276 protein (Myc Tag, His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

not tested

Cat no : Eg0075

Validation Data Gallery

  • Purity of Recombinant Human B7-H3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human B7-H3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human B7-H3 protein Leu29-Pro245 (Accession# Q5ZPR3-2) with a Myc tag and His tag at the C-terminus.
GeneID 80381
Accession Q5ZPR3-2
PredictedSize 28.3 kDa
SDS-PAGE 34-50 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

B7-H3 (CD276) is a type I transmembrane protein that belongs to the B7 immunoregulatory family. B7-H3 participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor. B7-H3 plays an essential role in cell proliferation, invasion, and migration in malignancies. Overexpressed in different types of human cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy.

References:

1.Yan R, et al. (2015). Inflammation. 38(3):1322-1328.
2.Zhang G, et al. (2010). J Immunol. 185(6):3677-3684.
3.Liu S, et al. (2021). Front Oncol. 11:654684.
4.Picarda E, et al. (2016). Clin Cancer Res. 22(14):3425-3431.