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Recombinant Human CD20 protein (His Tag)

Species

Human

Purity

>95 %, SDS-PAGE

Tag

His Tag

Activity

EC50: 10-40 ng/mL

Cat no : Eg0195

Validation Data Gallery

  • Purity of Recombinant Human CD20 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD20 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Rituximab at 0.5 μg/mL (100 μL/well) can bind Human CD20 (His tag) with a linear range of 10-40 ng/mL.

    Immobilized Rituximab at 0.5 μg/mL (100 μL/well) can bind Human CD20 (His tag) with a linear range of 10-40 ng/mL.

Product Information

Purity >95 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Immobilized Rituximab at 0.5 μg/mL (100 μL/well) can bind Human CD20 (His tag) with a linear range of 10-40 ng/mL.
Expression HEK293-derived Human CD20 protein Glu213-Pro297 (Accession# P11836-1) with a His tag at the N-terminus.
GeneID 931
Accession P11836-1
PredictedSize 10.6 kDa
SDS-PAGE 20-23 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny but not on either early pro-B cells or plasma blasts and plasma cells. It is found on B-cell lymphomas, hairy cell leukemia, B-cell chronic lymphocytic leukemia, and melanoma cancer stem cells. Its function is to enable optimal B-cell immune response, specifically against T-independent antigens. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation.

References:

1.Dong Fang. et al.(2005) Cancer Res. 65(20): 9328-37.
2.Pedersen M. et al.(2008) Biochem Biophys Res Commun. 377(1): 98-103.
3.Maria J Polyak. et al.(2008) J Biol Chem. 283(27): 18545-52.
4.Taco W Kuijpers. et al.(2010) J Clin Invest. 120(1): 214-22.
5.Gabriela Pavlasova. et al.(2018) Leukemia. 32(9): 2028-2031.