Recombinant Human VEGF121 protein (Myc tag, His tag)

• Home
• Products
• Fusion Proteins
• AuthentiKine ELISA Kits
• Recombinant Human VEGF121 protein (Myc tag, His tag) (Eg0192)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

Myc tag, His tag

Activity

EC50: 300-1230 ng/mL

Publications

1

Cat no : Eg0192

---

Validation Data Gallery

• 

  Purity of Recombinant Human VEGF121 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

• 

  Immobilized Human VEGF121 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human VEGFR1 (His tag) with a linear range of 300-1230 ng/mL.

---

Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <0.1 EU/μg protein, LAL method
• Activity:

  Immobilized Human VEGF121 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human VEGFR1 (His tag) with a linear range of 300-1230 ng/mL.

• Expression: HEK293-derived Human VEGF121 protein Ala27-Arg147 (Accession# P15692) with a Myc tag and a His tag at the C-terminus.
• GeneID: 7422
• Accession: P15692
• PredictedSize: 16.9 kDa
• SDS-PAGE: 20-24 kDa, reducing (R) conditions
• Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Vascular endothelial growth factor (VEGF), is a signal protein produced by cells that stimulates vasculogenesis and angiogenesis. It is part of the system that restores the oxygen supply to tissues when blood circulation is inadequate such as in hypoxic conditions. Serum concentration of VEGF is high in bronchial asthma and diabetes mellitus. The activities of VEGF are not limited to the vascular system; VEGF plays a role in normal physiological functions such as bone formation, hematopoiesis, wound healing, and development. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. VEGF is upregulated in many known tumors and its expression is correlated with tumor stage and progression. VEGF exists in several isoforms with different molecular weights and biological properties containing 121, 145, 165, 189, and 206 amino acids.

References:

1. Senger DR. et al. (1983). Science. 219: 983-5.
2. Ferrara N. et al. (1992). Endocr Rev. 13: 18-32.
3. Boocock CA. et al. (1995). J Natl Cancer Inst. 87: 506-516.
4. Sunderkotter C. et al. (1994). Int J Cancer. 55: 410-422.
5. T Cohen. et al. (1995) J Biol Chem. 270(19):11322-6.

Publications

Species	Title
human	Theriogenology; Endothelial cell-specific molecule 1 induced by interferon tau promotes angiogenesis in bovine endothelial cells.; Authors - Hayato Naito