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Lamin A/C Polyclonal antibody
Lamin A/C Polyclonal Antibody for FC (Intra), IF, IP, WB, ELISA
Cat no : 10298-1-AP
"Lamin A/C Antibodies" Comparison
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|Positive WB detected in||A431 cells, HEK-293 cells, HUVEC cells, mouse ovary tissue, SKOV-3 cells, HeLa cells, A375 cells, C6 cells, NIH/3T3 cells|
|Positive IP detected in||HeLa cells, A375 cells|
|Positive IF detected in||HepG2 cells, HeLa cells|
|Positive FC detected in||HEK-293T cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:20000-1:100000 for WB|
|Immunofluorescence (IF)||IF : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
10298-1-AP targets Lamin A/C in WB, IP, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, monkey, duck|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Lamin A/C fusion protein Ag0408|
|Full Name||lamin A/C|
|Calculated molecular weight||65 kDa|
|Observed molecular weight||65 kDa, 70 kDa|
|GenBank accession number||BC003162|
|Gene ID (NCBI)||4000|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Lamin A/C is also named as LMNA, or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.
|Product Specific Protocols|
|WB protocol for Lamin A/C antibody 10298-1-AP||Download protocol|
|IF protocol for Lamin A/C antibody 10298-1-AP||Download protocol|
|IP protocol for Lamin A/C antibody 10298-1-AP||Download protocol|
|FC protocol for Lamin A/C antibody 10298-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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SET1A-Mediated Mono-Methylation at K342 Regulates YAP Activation by Blocking Its Nuclear Export and Promotes Tumorigenesis.
XAF1 promotes anti-RNA virus immune responses by regulating chromatin accessibility
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Alejandro (Verified Customer) (08-22-2022)
Shows clear staining in IF and well staining using FACS
Charlotte (Verified Customer) (07-29-2022)
Cell fraction performed on NIH-3T3 cells to show the nucleus part. Blot super clean. Antibody specific. Easy to reveal.
S (Verified Customer) (12-31-2021)
Azita (Verified Customer) (06-16-2021)
It was used as a marker in WB to confirm proper nuclear fractionation of cell lysate.
Declan (Verified Customer) (11-29-2018)