Lamin A/C Polyclonal antibody

Lamin A/C Polyclonal Antibody for FC, IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, FC,ELISA

Conjugate

Unconjugated

Publications

91

Reviews (2)

Cat no : 10298-1-AP

Synonyms

70 kDa lamin, CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, lamin A, lamin A/C, LDP1, LFP, LGMD1B, LMN1, LMNA, LMNC, Prelamin A/C, PRO1, progerin

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• Validation Data Gallery

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• 

  WB analysis using 10298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10298-1-AP (Lamin A/C antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 10298-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10298-1-AP (Lamin A/C antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  WB analysis of NIH/3T3 using 10298-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP (Lamin A/C Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of HEK-293T using 10298-1-AP

  1X10^6 HEK-293T cells were stained with 0.2ug Lamin A/C antibody (10298-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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  IP experiment of A375 using 10298-1-AP

  IP Result of anti-lamin-A (IP:10298-1-AP, 3ug; Detection:10298-1-AP 1:1000) with A375 cells lysate 800ug.

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, HEK-293 cells, C6 cells, A375 cells, mouse ovary tissue, HUVEC cells, SKOV-3 cells
• Positive IP detected in: A375 cells
• Positive IF detected in: HepG2 cells, HeLa cells
• Positive FC detected in: HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 85 publications below
• IF: See 3 publications below

Product Information

10298-1-AP targets Lamin A/C in WB, IP, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: duck, human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Lamin A/C fusion protein Ag0408
• Full Name: lamin A/C
• Calculated molecular weight: 65 kDa
• Observed molecular weight: 65 kDa, 70 kDa
• GenBank accession number: BC003162
• Gene symbol: LMNA
• Gene ID (NCBI): 4000
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Lamin A/C is also named as LMNA, or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.

Publications

Species	Application	Title
human	WB	Cell Death Dis; Angiomotin-p130 inhibits β-catenin stability by competing with Axin for binding to tankyrase in breast cancer.; Authors - Jiao Yang
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Deficiency of heat shock protein A12A promotes browning of white adipose tissues in mice.; Authors - Hao Cheng
human		Front Microbiol; Inhibition of Influenza A Virus Replication by TRIM14 via Its Multifaceted Protein-Protein Interaction With NP.; Authors - Xiangwei Wu
mouse	WB	Mol Med Rep; miR‑494‑3p regulates lipopolysaccharide‑induced inflammatory responses in RAW264.7 cells by targeting PTEN.; Authors - Si Zhang
human	WB	Biochim Biophys Acta Gene Regul Mech; HSF1 phosphorylation by cyclosporin A confers hyperthermia sensitivity through suppression of HSP expression.; Authors - Jingyu Shao
human	WB	Toxicology; JNK activation-mediated nuclear SIRT1 protein suppression contributes to silica nanoparticle-induced pulmonary damage via p53 acetylation and cytoplasmic localisation.; Authors - Xinyuan Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Azita (Verified Customer) (06-16-2021)

It was used as a marker in WB to confirm proper nuclear fractionation of cell lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NSC-34 cell line

FH

Declan (Verified Customer) (11-29-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: Human brain homogenate

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