Lamin A/C Polyclonal antibody

Lamin A/C Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10298-1-AP

Synonyms

70 kDa lamin, CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, lamin A, lamin A/C, LDP1, LFP, LGMD1B, LMN1, LMNA, LMNC, Prelamin A/C, PRO1, progerin



Tested Applications

Positive WB detected inNIH/3T3 cells, HEK-293 cells, C6 cells, A375 cells, mouse ovary tissue, HUVEC cells, SKOV-3 cells
Positive IP detected inA375 cells
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10298-1-AP targets Lamin A/C in WB, IP, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity duck, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Lamin A/C fusion protein Ag0408
Full Name lamin A/C
Calculated molecular weight 65 kDa
Observed molecular weight 65 kDa, 70 kDa
GenBank accession numberBC003162
Gene symbol LMNA
Gene ID (NCBI) 4000
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lamin A/C is also named as LMNA, or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.

Protocols

Product Specific Protocols
WB protocol for Lamin A/C antibody 10298-1-APDownload protocol
IF protocol for Lamin A/C antibody 10298-1-APDownload protocol
IP protocol for Lamin A/C antibody 10298-1-APDownload protocol
FC protocol for Lamin A/C antibody 10298-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

Angiomotin-p130 inhibits β-catenin stability by competing with Axin for binding to tankyrase in breast cancer.

Authors - Jiao Yang
mouseWB

Biochim Biophys Acta Mol Basis Dis

Deficiency of heat shock protein A12A promotes browning of white adipose tissues in mice.

Authors - Hao Cheng
human

Front Microbiol

Inhibition of Influenza A Virus Replication by TRIM14 via Its Multifaceted Protein-Protein Interaction With NP.

Authors - Xiangwei Wu
mouseWB

Mol Med Rep

miR‑494‑3p regulates lipopolysaccharide‑induced inflammatory responses in RAW264.7 cells by targeting PTEN.

Authors - Si Zhang
humanWB

Biochim Biophys Acta Gene Regul Mech

HSF1 phosphorylation by cyclosporin A confers hyperthermia sensitivity through suppression of HSP expression.

Authors - Jingyu Shao
humanWB

Toxicology

JNK activation-mediated nuclear SIRT1 protein suppression contributes to silica nanoparticle-induced pulmonary damage via p53 acetylation and cytoplasmic localisation.

Authors - Xinyuan Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Declan (Verified Customer) (11-29-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/5000
  • Cell Tissue Type: Human brain homogenate