A375 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP(lamin-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

C6 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP( Lamin A/C Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP(lamin-A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 10298-1-AP( Lamin A/C Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 10298-1-AP(lamin-A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

mouse heart tissue were subjected to SDS PAGE followed by western blot with 10298-1-AP(lamin-A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10298-1-AP(lamin-A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP( Lamin A/C Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10298-1-AP(lamin-A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

IP Result of anti-lamin-A (IP:10298-1-AP, 3ug; Detection:10298-1-AP 1:1000) with A375 cells lysate 800ug.

This antibody specifically recognizes the heavy chain of Rabbit IgG. It can't detect the The heavy chain of other species. This antibody can be used as the secondary antibody which is specific to the heavy chain of Rabbit IgG.

Immunofluorescent analysis of HepG2 cells, using LMNA antibody 10298-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunofluorescent analysis of HepG2 cells using 10298-1-AP(Lamin A/C antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 10298-1-AP(Lamin A/C antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 10298-1-AP(Lamin A/C antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

1X10^6 HEK-293T cells were stained with 0.2ug Lamin A/C antibody (10298-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.