mDia1 Recombinant antibody

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  WB analysis using 85708-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85708-2-RR (mDia1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85708-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using mDia1 antibody (85708-2-RR, Clone: 243001A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85708-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85708-2-RR against Human mDia1 were performed. The affinity constant is 3.88 nM.

mDia1 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85708-2-RR

Clone No.243001A5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

DIAPH1, DIAP1, DIAPH 1, Diaphanous related formin 1, Diaphanous-related formin-1

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Tested Applications

• Positive WB detected in: HeLa cells, MDA-MB-231 cells, NIH/3T3 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85708-2-RR targets mDia1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: mDia1 fusion protein Ag14523
• Full Name: diaphanous homolog 1 (Drosophila)
• Calculated Molecular Weight: 1272 aa, 141 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC007411
• Gene Symbol: mDia1
• Gene ID (NCBI): 1729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60610
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

mDia1, also known as DIAPH1 or Diap1, is a mammalian diaphanous-related formin which is implicated in multiple physical and pathological events including cytoskeletal dynamics, autosomal hearing loss, and myelodysplasia. Depending upon the cell type and position in the cell cycle, mDia1 has been shown to localize to the cell cortex, trafficking endosomes, cleavage furrow, mid-bodies, and centrosomes, the cytoplasmic microtubule-organizing center crucial for cell division. Mutation of mDia1 has been linked to microcephaly. This antibody recognizes the endogenous mDia1 mainly around 140-150 kDa, while sometimes an additional 70 kDa can also be observed which is proposed to be a fragment of 140-150 kDa molecules (26011179).