ChromoTek mNeonGreen-Trap Magnetic Agarose

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Validation Data Gallery

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  Immunoprecipitation with mNeonGreen-Trap Magnetic Agarose: Coomassie and Western blot I: Input, FT: Flow-through, B: Bound.

The ChromoTek mNeonGreen-Trap Magnetic Agarose is an affinity resin for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to magnetic agarose beads.

Cat No. ntma

Publications(40)

Specificity

mNeonGreen

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Magnetic agarose

Synonyms

mNeonGreen-Trap, mNeonGreen, multimeric yellow fluorescence protein, mNeonGreen tag

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Product Information

The ChromoTek mNeonGreen-Trap Magnetic Agarose is an affinity resin for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to magnetic agarose beads.

• Description: Immunoprecipitation of mNeonGreen-fusion proteins and their interacting factors with anti-mNeonGreen Nanobody conjugated to magnetic agarose beads.

  • Fast, reliable & efficient one-step immunoprecipitation

  • Ready-to-use

  • No heavy & light antibody chains

  • Stable under harsh washing conditions

  • Suitable for downstream mass spec analysis

  • Works in samples from: mammals, plants, bacteria, yeast, insects etc.

• Applications: IP, CoIP, ChIP, RIP
• Specificity/Target: mNeonGreen; For the complete list, please click here: Fluorescent protein specificity table
• Binding capacity: 30 μg of recombinant mNeonGreen per 25 μL bead slurry
• Conjugate: Magnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Wash buffer compatibility: 4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 2 nM
• Compatibility with mass spectrometry: The mNeonGreen-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2827594
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!
• Size: 25ul/reactions (eg：20rxns=500ul slurry)

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Publications

Application	Title
ChIP	Nature; Ancient co-option of LTR retrotransposons as yeast centromeres.; Authors - Max A. B. Haase
IP	Nature; Polθ is phosphorylated by PLK1 to repair double-strand breaks in mitosis; Authors - Camille Gelot
	Nat Commun; Two distinct SWI/SNF complexes direct chromatin-linked transcriptional programs in Toxoplasma.; Authors - Dominic Schwarz
IP,CoIP	Nat Commun; Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex.; Authors - Dimitri Juillot
IP	Nat Commun; Positively charged specificity site in cyclin B1 is essential for mitotic fidelity; Authors - Christian Heinzle
CoIP	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex; Authors - Michael L van de Weijer