ChromoTek mStayGold-Trap Agarose, Kit for Immunoprecipitation

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• mStayGold-Trap Agarose, Kit for Immunoprecipitation (MG-3233AK)

Validation Data Gallery

• 

  Pulldown of spiked Strep-mStayGold, His-SUMO3-mStayGold2, and His-SUMO3-mBaoJin from HZ293S cell lysate by MG-3233A.
  5 µg of each antigen was spiked into lysate derived from 1.2*10^7 cells. For Coomassie and Western Blot analysis 1% of input (IN), 1% of flowthrough (F), and 20% of bound (B) fraction was loaded. His-SUMO3-mStayGold2 and His-SUMO3-mBaoJin were partially cleaved by intrinsic SUMO-proteases and cleaved mStayGold2 or mBaoJin is present in the bound fraction. Western Blots verify pulldown of Strep-mStayGold, His-SUMO3-mStayGold2 and His-SUMO3-mBaoJin.

• 

  Pulldown of StayGold, mBaoJin, and the fusion protein Aurora kinase B-mStayGold from transfected HEK 293T cells lysate by MG-3233A.
  Each IP was performed with lysate derived from 1x10^7 cells. A mock control with lysate from untransfected cells was included to demonstrate the low background binding of the resin. For Coomassie analysis of the SDS-gel 1% of input (IN), 1% of flowthrough (F), and 10% of bound (B) and Mock fraction was loaded.

The ChromoTek mStayGold-Trap Agarose, Kit for Immunoprecipitation consists of an anti-mStayGold VHH, which is coupled to agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of mStayGold or mBaoJin tagged proteins from cell extracts of various organisms.

Cat No. MG-3233AK

Reactivity

mStayGold, mBaoJin, mStayGold2, StayGold

Applications

IP, Co-IP

Host/Type

Alpaca, recombinant VHH

Conjugate

Agarose

Synonyms

mStayGold, mBaoJin, mStayGold2, StayGold

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Product Information

The ChromoTek mStayGold-Trap Agarose, Kit for Immunoprecipitation consists of an anti-mStayGold VHH, which is coupled to agarose beads. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of mStayGold or mBaoJin tagged proteins from cell extracts of various organisms.

• Description: Ready to use VHH coupled Agarose Beads for Immunoprecipitation of mStayGold.;

  • Fast, reliable and efficient one-step Immunoprecipitation;

  • Ready-to-use;

  • Stable in harsh buffer conditions;

  • Suitable for downstream mass spec analysis;

  • High Specificity;

  • Low background

• Applications: IP, Co-IP
• Reactivity: mStayGold, mBaoJin, mStayGold2, StayGold
• Binding Capacity: /
• Bead concentration: 50% Slurry
• Resin Type: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: 2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5
• Wash Buffer Compatibility: 1 M Urea, 1 M NaCl, 2% NP40, 5 mM β-mercaptoethanol, 2% Triton X-100, 5 mM TCEP, 5 mM DTT, 0.1% SDS
• Type: VHH
• Class: Recombinant - Animal free production
• Host: Alpaca
• Affinity: 280 nM (mStayGold) ; 240 nM (mBaoJin)
• Compatibility with mass spectrometry: /
• RRID: /
• Storage Buffer: 20% Ethanol
• Storage Condition: +4°C / do not freeze!
• Shipping: Ambient Temp

Kit components

• Component: Description
• mStayGold-Trap Agarose: 20 reactions (500 µl)
• Lysis buffer: Optimized for cytoplasmic proteins and mammalian cell lysis
• RIPA buffer: Optimized for nuclear/chromatin proteins and mammalian cell lysis
• Wash buffer: Removal of unwanted proteins, peptides, etc.
• Dilution buffer: Dilution of cell lysate
• Elution buffer: For acidic elution

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