p21 Monoclonal antibody

• 

  IF Staining of NIH/3T3 using 67362-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 67362-1-Ig (p21 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

p21 Monoclonal Antibody for IF/ICC, ELISA

Cat No. 67362-1-Ig

Clone No.3C7D3

Publications(12)

Host / Isotype

Mouse / IgG2b

Reactivity

mouse and More (2)

Applications

IF/ICC, ELISA

Synonyms

Cdkn1a, p21WAF, p21Cip1, P21 protein, Cdkn1

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Tested Applications

• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 7 publications below

Product Information

67362-1-Ig targets p21 in IF/ICC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28394

  Product name: Recombinant mouse Cdkn1a protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-159 aa of NM_001111099

  Sequence: MSNPGDVRPVPHRSKVCRCLFGPVDSEQLRRDCDALMAGCLQEARERWNFDFVTETPLEGNFVWERVRSLGLPKVYLSPGSRSRDDLGGDKRPSTSSALLQGPAPEDHVALSLSCTLVSERPEDSPGGPGTSQGRKRRQTSLTDFYHSKRRLVFCKRKP

• Full Name: cyclin-dependent kinase inhibitor 1A (P21)
• Calculated Molecular Weight: 18 kDa
• GenBank Accession Number: NM_001111099
• Gene Symbol: p21
• Gene ID (NCBI): 12575
• RRID: AB_2882614
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P39689
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human		J Transl Med; IP6K2 mutations as a novel mechanism of resistance to oncolytic virus therapy; Authors - Zhijian Huang
human,mouse		Front Pharmacol; Curcumol Suppresses CCF-Mediated Hepatocyte Senescence Through Blocking LC3B-Lamin B1 Interaction in Alcoholic Fatty Liver Disease.; Authors - Xiaoyu Qi
mouse		Life Sci; N6-methyladenosine modification on Hmbox1 is related to telomere dysfunction in DEHP-induced male reproductive injury; Authors - Xiaofang Zhu
canine	IF	Cell Prolif; TGF-β-induced PI3K/AKT/mTOR pathway controls myofibroblast differentiation and secretory phenotype of valvular interstitial cells through the modulation of cellular senescence in a naturally occurring in vitro canine model of myxomatous mitral valve disease; Authors - Qiyu Tang
mouse	IF	Heliyon; Mitochondrial citrate accumulation triggers senescence of alveolar epithelial cells contributing to pulmonary fibrosis in mice; Authors - Jie-Ru Hong
human,mouse	IF	Chemosphere; NBR1-dependent Autophagy Activation Protects Against Environmental Cadmium-evoked Placental Trophoblast Senescence; Authors - Qing Ling