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Mouse p21 Monoclonal antibody, PBS Only

p21 Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

mouse

Applications

IF, ELISA

Conjugate

Unconjugated

CloneNo.

3C7D3

Cat no : 67362-1-PBS

Synonyms

CAP20, CDKI, Cdkn1, Cdkn1a, CIP1, mda6, P21, p21Cip1, p21WAF, SDI1, Waf1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using mouse p21 Monoclonal antibody (67362-1-Ig)

    IF Staining of NIH/3T3 using 67362-1-Ig (same clone as 67362-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 67362-1-Ig (p21 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67362-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67362-1-PBS targets p21 in IF, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen p21 fusion protein Ag28394
Full Name cyclin-dependent kinase inhibitor 1A (P21)
Calculated Molecular Weight 18 kDa
GenBank Accession NumberNM_001111099
Gene Symbol Cdkn1a
Gene ID (NCBI) 12575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.