pan RAS Recombinant monoclonal antibody

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  WB analysis using 81615-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81615-1-RR (pan-RAS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 81615-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81615-1-RR (pan-RAS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 81615-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81615-1-RR (pan-RAS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 81615-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using pan-RAS antibody (81615-1-RR, Clone: 2I22 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  Affinity and Kinetic Characterization of 81615-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81615-1-RR against Human pan-RAS were performed. The affinity constant is below 1 pM.

pan RAS Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 81615-1-RR

Clone No.2I22

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

KRAS, 2I22, C K RAS, c Ki ras, c-Ki-ras

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, A549 cells, mouse brain tissue, rat brain tissue, pig liver tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

81615-1-RR targets pan RAS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2700

  Product name: Recombinant human KRAS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-188 aa of BC013572

  Sequence: MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM

• Full Name: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
• Calculated Molecular Weight: 188 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC013572
• Gene Symbol: KRAS
• Gene ID (NCBI): 3845
• RRID: AB_2935567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01116
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Front Immunol; NMB promotes the progression of colorectal cancer by regulating the NF-κB/P65 signaling pathway; Authors - Jiaxin Fan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (10-06-2025)

pan-RAS (in green) worked really nicely, appearing in the membrane of neurons and other cells in human brain FFPE tissue

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

pan RAS Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:81615-1-RR)