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HumanKine® recombinant human TPO (Thrombopoietin) protein (HZ-1248)

Validation Data Gallery

Purity of recombinant human TPO was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
Recombinant human TPO (HZ-1248) stimulates dose-dependent proliferation of the MO7e (human megakaryoblastic leukemia) cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells under defined media conditions were treated with increasing concentrations of recombinant TPO for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 100-500 ng/mL.
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Recombinant human TPO (HZ-1248) stimulates dose-dependent proliferation of the MO7e (human megakaryoblastic leukemia) cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. MO7e cells under defined media conditions were treated with increasing concentrations of recombinant TPO for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 100-500 ng/mL.
Human iPSC-derived microglia (white) residing within an in vivo brain-like organoid environment (blue) derived using HumanKine growth factors (TGF beta 1- HZ-1011, BMP4- HZ-1045, and Thrombopoietin HZ-1248).(Credits- Credit: Simon T. Schafer & Monique Pena, Technical University of Munich, Center for Organoid Systems)

HEK293 expressed
Endotoxin-free
Animal-component free

HumanKine® recombinant human TPO (Thrombopoietin) protein

Name: HumanKine® recombinant human TPO (Thrombopoietin) protein
Brand: Proteintech
SKU: HZ-1248
Rating: 5.00 (1 reviews)

Cat No. HZ-1248

Publications(15)

Reviews (1)

Activity(Primary)

100-500 ng/mL

Specific Activity

>1.00 x 10⁶ IU/mg

Species Reactivity

human

Purity

>95%

Synonyms

MGDF, MKCSF, ML, MPLLG, THPO, Thrombopoietin

Product type: Research use only

Research use only GMP

Size:

-/ -

Freight/Packing: -

Quantity

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Overview

Technical Specifications
Stability and Reconstitution
Product Description
Publications (15)
FAQs
Reviews (1)

Print datasheet

Download Certificate of Analysis

MSDS

Technical Specifications

GeneID	7066
Species	Human
Expression	HEK293
Activity(Primary)	100-500 ng/mL
Activity(Secondary)	1-7 ng/mL EC50 in MO7e cells
Specific Activity	>1.00 x 10⁶ IU/mg
Purity	>95%
Endotoxin	< 1 EU/µg
Accession Number	P40225
Molecular Mass	70 to 93 kDa reduced and non-reduced, monomer, glycosylated.
Formulation	1x PBS, See Certificate of Analysis for details
Species Reactivity	human

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

TPO (Thrombopoietin) is a primary regulator of megakaryocyte development and platelet production in mammals. Human Thrombopoietin constitutively circulates and maintains thrombopoiesis through interaction with its cognate receptor, myeloproliferative leukemia protein(MPL). Thrombopoietin also plays an important role in the maintenance and regulation of hematopoietic stem cells (HSCs).

Synonyms

MGDF, MKCSF, ML, MPLLG, THPO, Thrombopoietin

Publications

Species	Title
human	Cell An in vivo neuroimmune organoid model to study human microglia phenotypes Authors - Simon T Schafer View Article
human	Blood PF4 activates the c-Mpl-Jak2 pathway in platelets Authors - Richard John Buka View Article
human	Nucleic Acids Res Donor insertion into CX3CR1 allows epigenetic modulation of a constitutive promoter on hematopoietic stem cells and its activation upon myeloid differentiation Authors - Iris Ramos-Hernández View Article
human	J Immunother Cancer Targeting endosomal trafficking-mediated antigen escape to resensitize myeloma to CAR-T therapy. Authors - Zhuning Wang View Article
human	J Exp Med Co-inhibition of NF-κB and JNK is synergistic in TNF-expressing human AML. Authors - AndrewVolk View Article
human	Oncogene β-Catenin interacts with canonical RBPs including MSI2 to associate with a Wnt signalling mRNA network in myeloid leukaemia cells Authors - M Wagstaff View Article