• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human BMP-2 protein

Activity

7.5-37.5 ng/mL EC50

Specific Activity

7.2 x 10^5 IU/mg calibrated against BMP-2 reference standard (NIBSC cat no: 93/574). Reference use only. Specific activity is not routinely tested

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1128

Synonyms

BMP 2, BMP 2A, BMP2, BMP-2, BMP2A, bone morphogenetic protein 2, Bone morphogenetic protein 2A



Technical Specifications

GeneID 650
Species Human
Expression HEK293
Activity 7.5-37.5 ng/mL EC50
Specific Activity7.2 x 10^5 IU/mg calibrated against BMP-2 reference standard (NIBSC cat no: 93/574). Reference use only. Specific activity is not routinely tested
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP21274
Molecular Mass 28 kDa, homodimer, glycosylated
Formulation 2x PBS + 6% Ethanol, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP-2 induces bone formation and regeneration during early embryonic development (PMID 16234975). It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction (PMID 26620161). Xeno-free Recombinant Human BMP-2 is expressed from human 293 cells as a disulfide linked homodimeric glycoprotein with an apparent molecular mass of 28 kDa.

Synonyms

BMP 2, BMP 2A, BMP2, BMP-2, BMP2A, bone morphogenetic protein 2, Bone morphogenetic protein 2A

Publications

SpeciesTitle
rat

Adv Mater

Titanium Fiber Plates for Bone Tissue Repair.

Authors - Takashi Takizawa
human,mouse

Nat Commun

Deficiency of TMEM53 causes a previously unknown sclerosing bone disorder by dysregulation of BMP-SMAD signaling.

Authors - Long Guo
human

J Exp Med

Aberrant ZNF423 impedes B cell differentiation and is linked to adverse outcome of ETV6-RUNX1 negative B precursor acute lymphoblastic leukemia.

Authors - LenaHarder
human

Biomaterials

Dual growth factor loaded nonmulberry silk fibroin/carbon nanofiber composite 3D scaffolds for in vitro and in vivo bone regeneration.

Authors - DebokiNaskar
mouse

J Cell Biol

Pannexin 3 functions as an ER Ca(2+) channel, hemichannel, and gap junction to promote osteoblast differentiation.

Authors - MasakiIshikawa

Acta Biomater

Effect of calcium phosphate coating and rhBMP-2 on bone regeneration in rabbit calvaria using poly(propylene fumarate) scaffolds.

Authors - MahrokhDadsetan