Validation Data Gallery
Technical Specifications
| GeneID | 3552 |
| Species | Human |
| Expression | HEK293 |
| Activity(Primary) | 0.125-1.25 ng/mL in D10.G4.1 Cells |
| Activity(Secondary) | 8-40 ng/mL in an HEK293 reporter cell line |
| Purity | >95% |
| Endotoxin | <1 EU/µg |
| Accession Number | P01583 |
| Molecular Mass | 22 kDa reduced and non-reduced, monomer, glycosylated |
| Formulation | 1x PBS, See Certificate of Analysis for details |
| Species Reactivity | human,mouse |
Stability and Reconstitution
| Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
|---|---|---|---|
| Lyophilized | -20°C to -80°C | Until Expiry Date | |
| Lyophilized | Room Temperature | 2 weeks | |
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |
| Reconstituted as per CofA | 4°C | 1 week | |
| Avoid repeated freeze-thaw cycles. | |||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
Background
IL-1 alpha is a dual function cytokine that can function both as a transcription factor and a damage-associated molecular pattern (DAMP) in innate immune responses. It is expressed by many cell types and its expression is increased when cells are exposed to growth factors, pro-inflammatory, and stress-related signals. In its pro-form, IL-1 alpha contains a nuclear localization signal that allows it to translocate to the nucleus and bind to DNA, resulting in either the increase or prohibition of pro-inflammatory cytokine expression. When cells are exposed to necrotic signals, mature IL-1 alpha is released from the cell lysates and triggers a strong pro-inflammatory response upon binding to its receptor on nearby cells. Due to its strongly pro-inflammatory nature, IL-1 alpha has been linked to tumor metastasis and lower median survival in several cancers (PMID: 27434011, 29247995).
Synonyms
Hematopoietin 1, Pro-interleukin-1-alpha, IL1 alpha, IL1, IL1A, IL-1A, IL1-ALPHA, IL1F1, IL-1F1, preinterleukin 1 alpha, IL1F1hematopoietin-1, interleukin 1, alpha, interleukin-1 alpha, LAF, LEM
Publications
| Species | Title |
|---|---|
NPJ Precis Oncol An integrated single-cell lung cancer atlas reveals distinct fibroblast phenotypes between adenocarcinoma and squamous cell carcinomas. | |
Biochem Biophys Rep CEACAM5 drives psoriatic inflammation by regulating CD8(+) T cell recruitment and activation. | |
Invest Ophthalmol Vis Sci Ocular IL-1α/IFN-γ Delay the Corneal Wound Healing in Rheumatoid Arthritis Through Mitochondrial Dysfunction and NLRP3 Inflammasome Activation. | |
Int Immunopharmacol IL-19 promotes psoriasis progression by inhibiting keratinocyte apoptosis via the Bcl-2/Caspase-3 axis. | |
Signal Transduct Target Ther PRELP negatively regulates IL-17A-mediated proliferation and the inflammatory response in psoriasis. | |
Skin Res Technol Livin expression promotes keratinocyte release of inflammatory mediators in psoriasis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Margarida Rodrigues (Verified Customer) (08-03-2026) | The HZ-1320 recombinant protein performed very well in our experiments. It showed excellent biological activity, high purity, and consistent results across our assays. The protein was easy to reconstitute and remained stable when handled according to the recommended protocol. Overall, the product met our expectations and proved to be a reliable reagent for cell signaling and inflammation research. I would recommend it for research applications.
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FH Emmanuel (Verified Customer) (11-10-2025) | Works in NF-kappaB induction using luciferase reporter assay
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