Validation Data Gallery
Technical Specifications
| GeneID | 9241 |
| Species | Human |
| Expression | HEK293 |
| Activity(Primary) | 1.5-15 ng/mL |
| Purity | >95% |
| Endotoxin | <1 EU/μg |
| Accession Number | Q13253 |
| Molecular Mass | 28 kDa reduced, 50 to 95 kDa non-reduced, homodimer, glycosylated |
| Formulation | 1x PBS, See Certificate of Analysis for details |
| Species Reactivity | human,mouse |
Stability and Reconstitution
| Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
|---|---|---|---|
| Lyophilized | -20°C to -80°C | Until Expiry Date | |
| Lyophilized | Room Temperature | 2 weeks | |
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |
| Reconstituted as per CofA | 4°C | 1 week | |
| Avoid repeated freeze-thaw cycles. | |||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
Background
Recombinant human Noggin protein is widely used in stem cell maintenance and directed differentiation, particularly in iPSC and embryonic stem cell systems where precise control of cell fate is essential. It also plays an important role in organoid differentiation, supporting the development of complex 3D tissue models. HumanKine Noggin is produced using a HEK293 expression system, ensuring a biologically active and structurally accurate form of the native human protein for consistent performance in demanding cell culture applications. Noggin is a secreted dimeric protein that functions as a high-affinity inhibitor of bone morphogenetic proteins (BMPs), blocking receptor interaction and downstream signaling. By suppressing BMP pathways, Noggin plays a critical role in neural differentiation, early developmental processes, and controlled pluripotent stem cell progression, supporting applications in regenerative medicine and advanced cell-based models. (PMID: 29497255; 29167826; 29094227; 28842965).
Synonyms
NOG, noggin, SYM1, SYNS1
Publications
| Species | Title |
|---|---|
Cell Stem Cell Increased Neural Progenitor Proliferation in a hiPSC Model of Autism Induces Replication Stress-Associated Genome Instability. | |
Nat Methods Long-term expansion of alveolar stem cells derived from human iPS cells in organoids. | |
Cell Stem Cell Efficient Generation of CA3 Neurons from Human Pluripotent Stem Cells Enables Modeling of Hippocampal Connectivity In Vitro. | |
Nat Commun Pharmacological blockade of TEAD–YAP reveals its therapeutic limitation in cancer cells | |
Biomaterials Core-shell hydrogel microfiber-expanded pluripotent stem cell-derived lung progenitors applicable to lung reconstruction in vivo. | |
Curr Biol Human-specific regulation of neural maturation identified by cross-primate transcriptomics |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Dan (Verified Customer) (02-27-2026) | successfully supported colorectal cancer organoid culture
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FH Evelyne (Verified Customer) (11-20-2025) | This Noggin supplement is very good for organoid culture. Cells grew well and were very happy
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FH Lindsay (Verified Customer) (09-03-2024) | Recombinant human noggin is being used to generate tumouroids from patient tumour tissue. The cultures look successful and the supplier is one of the most competitively priced around. Would recommend.
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FH Lisa (Verified Customer) (08-11-2022) | The cytokine works well, with results comparable with the ones observed using the same product from other companies.
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