Validation Data Gallery

Purity of recombinant human TGF beta 2 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.
GMP Recombinant human TGF beta 2 (HZ-1092-GMP) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of GMP recombinant TGF beta 2 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 0.018-0.18 ng/mL.
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GMP Recombinant human TGF beta 2 (HZ-1092-GMP) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of GMP recombinant TGF beta 2 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 0.018-0.18 ng/mL.

HEK293 expressed
Endotoxin-free
Animal-component free

HumanKine® recombinant human TGF beta 2 protein- GMP grade

Name: HumanKine® recombinant human TGF beta 2 protein- GMP grade
Brand: Proteintech
SKU: HZ-1092-GMP

Cat No. HZ-1092-GMP

Review Product

EC50

0.018-0.18 ng/mL

Specific Activity

minimally 2.00 x 10⁷ IU/mg, typically 3.52 x 10⁷ IU/mg

Species Reactivity

human,mouse

Purity

>95%

Synonyms

BSC 1 cell growth inhibitor, Cetermin, G TSF, Polyergin, TGF beta 2, TGF beta2, TGFB2

Product type: GMP

Research use only GMP

Size:

-/ -

Freight/Packing: -

Quantity

- +

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GMP-Grade HumanKine® Info

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Overview

Technical Specifications
Stability and Reconstitution
GMP Quality Policies
Product Description
FAQs

Print datasheet

Download Certificate of Analysis

MSDS

Technical Specifications

GeneID	7042
Species	Human
Expression	HEK293
EC50	0.018-0.18 ng/mL
Specific Activity	minimally 2.00 x 10⁷ IU/mg, typically 3.52 x 10⁷ IU/mg
Purity	>95%
Endotoxin	<0.1 EU/µg
Accession Number	P61812
Molecular Mass	13 kDa reduced, 23 kDa non-reduced, homodimer, non-glycosylated
Formulation	50mM Acetate pH 3.0 + 20% EtOH, See Certificate of Analysis for details
Species Reactivity	human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 20% ethanol + 50 mM NaOAc + 75 mM HOAc. Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Recombinant Human Transforming Growth Factor Beta-2 (TGF beta-2) is a multifunctional cytokine that plays a critical role in cardiac development, cell proliferation, and differentiation. It is widely used in the expansion and maintenance of pluripotent human embryonic stem cells (hESCs), making it essential for stem cell research, regenerative medicine, and cell and gene therapy (CGT) workflows. HumanKine TGF beta-2 is expressed in HEK293 human cells, ensuring authentic human post-translational modifications (PTMs) that enhance protein folding, stability, and biological activity compared to non-mammalian expression systems. TGF beta-2 functions by binding to type I and type II TGF beta receptors, activating SMAD signaling pathways that regulate gene expression and control cell fate decisions. Manufactured under ISO 13485-compliant systems, it is animal component-free, xeno-free, and endotoxin-controlled for GMP-aligned applications.

Synonyms