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What's the best dilution for scFv Isotype Control in ?
Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-SF98136 targets scFv Isotype Control in FC applications and shows reactivity with n/a samples.
| Tested Reactivity | n/a |
| Host / Type | Rabbit / scFv |
| Class | Recombinant |
| Type | Single-chain variable fragment antibody (scFv) |
| Immunogen |
Small molecule Predict reactive species |
| Full Name | scFv Isotype Control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| Conjugate | CoraLite® Plus 594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Excitation Laser | Yellow-Green Laser (561 nm) |
| Source | Rabbit scFv isotype control from clone 240953C9, consisting of VL-GGGGS linker-VH (N- to C-terminus), with a His tag at the C-terminus. |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin300, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul sizes contain 0.1% BSA. |
Background Information
This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 594 scFv Isotype Control antibody CL594-SF98136 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
