smooth muscle actin specific Monoclonal antibody
smooth muscle actin specific Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 67735-1-Ig
Validation Data Gallery
|Positive WB detected in||rat stomach tissue, pig vein tissue, pig stomach tissue, rat colon, mouse colon, pig colon|
|Positive IHC detected in||human colon tissue, human appendicitis tissue, human liver tissue, human liver cancer tissue, rat colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||rat heart tissue|
|Western Blot (WB)||WB : 1:20000-1:100000|
|Immunohistochemistry (IHC)||IHC : 1:2500-1:10000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
67735-1-Ig targets smooth muscle actin specific in WB, IHC, IF applications and shows reactivity with Human, mouse, rat, pig samples.
|Tested Reactivity||Human, mouse, rat, pig|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Full Name||actin, alpha 2, smooth muscle, aorta|
|Calculated molecular weight||42 kDa|
|Observed molecular weight||42 kDa|
|GenBank accession number||NM_001613|
|Gene ID (NCBI)||59|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.
|Product Specific Protocols|
|WB protocol for smooth muscle actin specific antibody 67735-1-Ig||Download protocol|
|IHC protocol for smooth muscle actin specific antibody 67735-1-Ig||Download protocol|
|IF protocol for smooth muscle actin specific antibody 67735-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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