smooth muscle actin specific Monoclonal antibody

smooth muscle actin specific Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human, mouse, rat, pig, rabbit and More (2)







Cat no : 67735-1-Ig


a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, sma, smooth muscle actin, smooth muscle actin specific, α-sma

Tested Applications

Positive WB detected inrat stomach tissue, rabbit stomach tissue, pig vein tissue, pig stomach tissue, rabbit large intestine tissue, pig colon tissue, rat colon tissue, mouse colon tissue
Positive IHC detected inhuman colon tissue, human appendicitis tissue, human liver tissue, human liver cancer tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat heart tissue, C2C12 cells
Positive FC detected inC2C12 cells

Recommended dilution

Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67735-1-Ig targets smooth muscle actin specific in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Cited Reactivityhuman, mouse, rat, zebrafish, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name actin, alpha 2, smooth muscle, aorta
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberNM_001613
Gene Symbol ACTA2
Gene ID (NCBI) 59
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.


Product Specific Protocols
WB protocol for smooth muscle actin specific antibody 67735-1-IgDownload protocol
IHC protocol for smooth muscle actin specific antibody 67735-1-IgDownload protocol
IF protocol for smooth muscle actin specific antibody 67735-1-IgDownload protocol
FC protocol for smooth muscle actin specific antibody 67735-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Adv Mater

A "Bridge-Building" Glycan Scaffold Mimicking Microbial Invasion for In Situ Endothelialization.

Authors - Ruoyu Mu

Mol Cancer

circFARP1 enables cancer-associated fibroblasts to promote gemcitabine resistance in pancreatic cancer via the LIF/STAT3 axis.

Authors - Chonghui Hu

Adv Sci (Weinh)

Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Esophageal Squamous Cell Carcinoma

Authors - Yongxu Jia

Acta Pharm Sin B

ADAR1 regulates vascular remodeling in hypoxic pulmonary hypertension through N1-methyladenosine modification of circCDK17

Authors - Junting Zhang


Extended-release of therapeutic microRNA via a host-guest supramolecular hydrogel to locally alleviate renal interstitial fibrosis.

Authors - Yingying Xu


Inhibition of PLA2G4E/cPLA2 promotes survival of random skin flaps by alleviating Lysosomal membrane permeabilization-Induced necroptosis.

Authors - Junsheng Lou