CoraLite®647-conjugated smooth muscle actin specific Monoclonal antibody

smooth muscle actin specific Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

IF

Conjugate

CoraLite®647 Fluorescent Dye

CloneNo.

1E9A11

Cat no : CL647-67735

Synonyms

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA



Tested Applications

Positive IF detected inmouse heart tissue
For other applications, we recommend the unconjugated version of this antibody, 67735-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL647-67735 targets smooth muscle actin specific in IF applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name actin, alpha 2, smooth muscle, aorta
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberNM_001613
Gene symbol ACTA2
Gene ID (NCBI) 59
Conjugate CoraLite®647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm/665 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

Protocols

Product Specific Protocols
IF protocol for CL647 smooth muscle actin specific antibody CL647-67735Download protocol
Standard Protocols
Click here to view our Standard Protocols