smooth muscle actin specific Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

smooth muscle actin specific Recombinant Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, chicken, pig, zebrafish

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5H7

Cat no : 80008-1-PBS

Synonyms

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, SMA, smooth muscle actin, smooth muscle actin specific, smooth muscle actin-specific

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Validation Data Gallery

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  WB analysis using 80008-1-RR (same clone as 80008-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80008-1-RR (same clone as 80008-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 80008-1-RR (same clone as 80008-1-PBS)

  WB result of smooth muscle actin specific antibody (80008-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-smooth muscle actin specific transfected A549 cells. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

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  WB analysis of zebrafish using 80008-1-RR (same clone as 80008-1-PBS)

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80008-1-RR (same clone as 80008-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 80008-1-RR (same clone as 80008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 80008-1-RR (same clone as 80008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 80008-1-RR (same clone as 80008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 80008-1-RR (same clone as 80008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 80008-1-RR (same clone as 80008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 80008-1-RR (same clone as 80008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 80008-1-RR (same clone as 80008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 80008-1-RR (same clone as 80008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 80008-1-RR (same clone as 80008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 80008-1-RR (same clone as 80008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 80008-1-RR (same clone as 80008-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 80008-1-RR (same clone as 80008-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 80008-1-RR (smooth muscle actin specific antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse embryo using 80008-1-RR (same clone as 80008-1-PBS)

  E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

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  FC experiment of C2C12 using 80008-1-RR (same clone as 80008-1-PBS)

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human smooth muscle actin specific (80008-1-RR, Clone:5H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80008-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80008-1-PBS targets smooth muscle actin specific in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, chicken, pig, zebrafish samples.

• Tested Reactivity: Human, mouse, rat, chicken, pig, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: actin, alpha 2, smooth muscle, aorta
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_001613
• Gene Symbol: ACTA2
• Gene ID (NCBI): 59
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.