smooth muscle actin specific Recombinant antibody

smooth muscle actin specific Recombinant Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat, chicken, pig

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

5H7

Cat no : 80008-1-RR

Synonyms

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, SMA



Tested Applications

Positive WB detected inmouse colon tissue, pig vein tissue, chicken crop tissue, pig stomach, pig colon, rat stomach, chicken small intestine
Positive IHC detected inhuman liver tissue, human heart tissue, human appendicitis tissue, mouse colon tissue, rat colon tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue, mouse embryo tissue
Planning an IHC experiment? We recommend our IHCeasy smooth muscle actin Ready-To-Use IHC Kit. smooth muscle actin primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

80008-1-RR targets smooth muscle actin specific in WB, IHC, IF,ELISA applications and shows reactivity with Human, mouse, rat, chicken, pig samples.

Tested Reactivity Human, mouse, rat, chicken, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name actin, alpha 2, smooth muscle, aorta
Calculated molecular weight 42 kDa
GenBank accession numberNM_001613
Gene symbol ACTA2
Gene ID (NCBI) 59
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

Protocols

Product Specific Protocols
WB protocol for smooth muscle actin specific antibody 80008-1-RRDownload protocol
IHC protocol for smooth muscle actin specific antibody 80008-1-RRDownload protocol
IF protocol for smooth muscle actin specific antibody 80008-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols