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  • Western Blot (WB) analysis of various lysates using ACAA2 Recombinant antibody (86161-3-RR)

    WB analysis using 86161-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86161-3-RR (ACAA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ACAA2 Recombinant antibody (86161-3-RR)

    IF Staining of HepG2 using 86161-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACAA2 antibody (86161-3-RR, Clone: 250814D2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86161-3-RR

    Affinity and Kinetic Characterization of 86161-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86161-3-RR against Human ACAA2 were performed. The affinity constant is 0.598 nM.

ACAA2 Recombinant monoclonal antibody

ACAA2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86161-3-RR
Clone No.250814D2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

3-ketoacyl-CoA thiolase, mitochondrial, Acetyl CoA acyltransferase, Acetyl-CoA acetyltransferase, Acetyl-CoA acyltransferase, Acyl-CoA hydrolase, mitochondrial

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, Ramos cells, Caco-2 cells, NIH/3T3 cells, HSC-T6 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86161-3-RR targets ACAA2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1609

Product name: Recombinant human ACAA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 98-397 aa of BC001918

Sequence: SIVNGCQEICVKEAEVVLCGGTESMSQAPYCVRNVRFGTKLGSDIKLEDSLWVSLTDQHVQLPMAMTAENLAVKHKISREECDKYALQSQQRWKAANDAGYFNDEMAPIEVKTKKGKQTMQVDEHARPQTTLEQLQKLPPVFKKDGTVTAGNASGVADGAGAVIIASEDAVKKHNFTPLARIVGYFVSGCDPSIMGIGPVPAISGALKKAGLSLKDMDLVEVNEAFAPQYLAVERSLDLDISKTNVNGGAIALGHPLGGSGSRITAHLVHELRRRGGKYAVGSACIGGGQGIAVIIQSTA

Predict reactive species
Full Name acetyl-Coenzyme A acyltransferase 2
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC001918
Gene Symbol ACAA2
Gene ID (NCBI) 10449
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP42765
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ACAA2 antibody 86161-3-RRDownload protocol
IF protocol for ACAA2 antibody 86161-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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