CoraLite® Plus 488-conjugated ACAA2 Recombinant monoclonal antibody

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  IF Staining of HepG2 using CL488-86161-3

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 ACAA2 antibody (CL488-86161-3, Clone: 250814D2 ) at dilution of 1:200, CL594-phalloidin (red).

ACAA2 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-86161-3

Clone No.250814D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

3-ketoacyl-CoA thiolase, mitochondrial, Acetyl CoA acyltransferase, Acetyl-CoA acetyltransferase, Acetyl-CoA acyltransferase, Acyl-CoA hydrolase, mitochondrial

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-86161-3 targets ACAA2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1609

  Product name: Recombinant human ACAA2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 98-397 aa of BC001918

  Sequence: SIVNGCQEICVKEAEVVLCGGTESMSQAPYCVRNVRFGTKLGSDIKLEDSLWVSLTDQHVQLPMAMTAENLAVKHKISREECDKYALQSQQRWKAANDAGYFNDEMAPIEVKTKKGKQTMQVDEHARPQTTLEQLQKLPPVFKKDGTVTAGNASGVADGAGAVIIASEDAVKKHNFTPLARIVGYFVSGCDPSIMGIGPVPAISGALKKAGLSLKDMDLVEVNEAFAPQYLAVERSLDLDISKTNVNGGAIALGHPLGGSGSRITAHLVHELRRRGGKYAVGSACIGGGQGIAVIIQSTA

• Full Name: acetyl-Coenzyme A acyltransferase 2
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC001918
• Gene Symbol: ACAA2
• Gene ID (NCBI): 10449
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P42765
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.