ACE2 Antibody

ACE2 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2F12A4

Cat no : 66699-1-Ig

Synonyms

ACE related carboxypeptidase, ACE2, ACEH, DKFZp434A014, Metalloprotease MPROT15



Tested Applications

Positive WB detected inhuman testis tissue, human skeletal muscle tissue
Positive IHC detected inhuman small intestine tissue, human testis tissue, human kidney tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inNIH/3T3 cells, HeLa cells, THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

66699-1-Ig targets ACE2 in WB, IHC, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ACE2 fusion protein Ag15554
Full Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Calculated molecular weight 805aa,92 kDa
Observed molecular weight 120 kDa, 92 kDa
GenBank accession number BC048094
Gene symbol ACE2
Gene ID (NCBI) 59272
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2. The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088).

Protocols

Product Specific Protocols
WB protocol for ACE2 antibody 66699-1-IgDownload protocol
IHC protocol for ACE2 antibody 66699-1-IgDownload protocol
FC protocol for ACE2 antibody 66699-1-IgDownload protocol
Standard Protocols
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