Tested Applications
| Positive WB detected in | human heart tissue, pig heart tissue, rat heart tissue, mouse heart tissue |
| Positive IHC detected in | human heart tissue, human heart and human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF-Fro detected in | mouse heart tissue |
| Positive FC (Intra) detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2500-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66125-1-Ig targets Alpha cardiac muscle actin specific in WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | actin, alpha, cardiac muscle 1 |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | NM_005159 |
| Gene Symbol | ACTC1 |
| Gene ID (NCBI) | 70 |
| RRID | AB_2881524 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P68032 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Alpha cardiac muscle actin specific antibody 66125-1-Ig | Download protocol |
| IF protocol for Alpha cardiac muscle actin specific antibody 66125-1-Ig | Download protocol |
| IHC protocol for Alpha cardiac muscle actin specific antibody 66125-1-Ig | Download protocol |
| WB protocol for Alpha cardiac muscle actin specific antibody 66125-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-ACTC1 antibody (Cat# 66125-1-Ig) has accumulated 16 citations across journals including J Clin Invest, Cell Mol Life Sci, Life Sciences, J Am Heart Association, European Journal of Pharmacology, Development, International Journal of Cardiology, Frontiers in Molecular Biosciences, and PLoS One. Research fields are predominantly cardiology—covering cardiomyopathy, myocardial infarction, heart failure, and cardiac development—with additional studies in oncology, immunology, toxicology, and atherosclerosis.
| Species | Application | Title |
|---|---|---|
J Clin Invest Frameshift variants in C10orf71 cause dilated cardiomyopathy in human, mouse, and organoid models | ||
Antioxidants (Basel) Rhynchophylline Protects Against Ischemic Injury Following Myocardial Infarction via Activation of the SIRT1/NRF2/FOXO3a Axis. | ||
J Ethnopharmacol Qishen paste improves cardiac conduction in heart failure by regulating cardiac resident macrophage Connexin43. | ||
Cell Mol Life Sci AICAr Inhibition of cardiomyocyte autophagy promotes p62-dependent NRF2 expression and protection against doxorubicin toxicity. | ||
Life Sci Cadmium exposure induces a sex-dependent decline in left ventricular cardiac function | ||
J Am Heart Assoc Postinfarction Hearts Are Protected by Premature Senescent Cardiomyocytes Via GATA 4-Dependent CCN 1 Secretion. |
Reviews
What customers say
The single review for 66125-1-Ig is from Marco Neu (July 2024), who rated it 5 stars. He reported that the antibody "worked very well" for Western Blot and Immunofluorescence on NRVCMs (neonatal rat ventricular cardiomyocytes), using dilutions of 1:1000 and 1:2000 with lot 10003773. Overall, the feedback is positive, indicating reliable performance across both applications at the tested dilutions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marco (Verified Customer) (07-03-2024) | Worked very well for us
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