CoraLite® Plus 488-conjugated ACTC1-specific Monoclonal antibody

ACTC1-specific Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F2B9

Cat no : CL488-66125

Synonyms

ACTC, ACTC1, ACTC1 cardiac muscle 1, ACTC1-specific, Actin, alpha cardiac muscle 1, actin, alpha, cardiac muscle 1, Alpha cardiac actin, CMD1R, CMH11

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Validation Data Gallery

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  IF Staining of mouse heart using CL488-66125

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CL488-66125 (ACTC1-specific antibody) at dilution of 1:100.

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  IF Staining of mouse heart using CL488-66125

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CL488-66125 (ACTC1-specific antibody) at dilution of 1:100.

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Tested Applications

• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66125 targets ACTC1-specific in IF-P applications and shows reactivity with human, mouse, pig, rat samples.

• Tested Reactivity: human, mouse, pig, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: actin, alpha, cardiac muscle 1
• Calculated Molecular Weight: 42 kDa
• GenBank Accession Number: NM_005159
• Gene Symbol: ACTC1
• Gene ID (NCBI): 70
• RRID: AB_2883266
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.