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ACVR1 Monoclonal antibody

ACVR1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Mouse, Rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1F11B10

Cat no : 67417-1-Ig

Synonyms

activin A receptor, type I, Activin receptor like kinase 2, Activin receptor type 1, Activin receptor type I, ACTR I, ACTRI, ACVR1, ACVR1A, ACVRLK2, ALK 2, ALK2, FOP, SKR1, TSR I, TSRI

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ACVR1 Monoclonal antibody (67417-1-Ig)

    WB analysis using 67417-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67417-1-Ig (ACVR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using ACVR1 Monoclonal antibody (67417-1-Ig)

    WB analysis of rat brain using 67417-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67417-1-Ig (ACVR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using ACVR1 Monoclonal antibody (67417-1-Ig)

    WB analysis of pig brain using 67417-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67417-1-Ig (ACVR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ACVR1 Monoclonal antibody (67417-1-Ig)

    WB analysis using 67417-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67417-1-Ig (ACVR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using ACVR1 Monoclonal antibody (67417-1-Ig)

    IHC staining of mouse heart using 67417-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67417-1-Ig (ACVR1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using ACVR1 Monoclonal antibody (67417-1-Ig)

    IHC staining of mouse heart using 67417-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67417-1-Ig (ACVR1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ACVR1 Monoclonal antibody (67417-1-Ig)

    IHC staining of mouse brain using 67417-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67417-1-Ig (ACVR1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ACVR1 Monoclonal antibody (67417-1-Ig)

    IHC staining of mouse brain using 67417-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67417-1-Ig (ACVR1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using ACVR1 Monoclonal antibody (67417-1-Ig)

    IF Staining of mouse brain using 67417-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ACVR1 antibody (67417-1-Ig, Clone: 1F11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using ACVR1 Monoclonal antibody (67417-1-Ig)

    IF Staining of mouse brain using 67417-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ACVR1 antibody (67417-1-Ig, Clone: 1F11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig brain tissue, rat brain tissue, NCI-H1299 cells, mouse brain tissue, JAR cells
Positive IHC detected inmouse heart tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67417-1-Ig targets ACVR1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Reactivity Human, Pig, Mouse, Rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ACVR1 fusion protein Ag13508
Full Name activin A receptor, type I
Calculated Molecular Weight 509 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC033867
Gene Symbol ACVR1
Gene ID (NCBI) 90
RRIDAB_2882657
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACVR1 (activin receptor type I), also known as ALK2 or ACTRI, is a receptor for activin. It forms a stable complex with type II receptor after ligand binding. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling, and type II receptors are required for binding ligands and for expression of type I receptors. ACVR1 is expressed in many tissues including skeletal muscle and chondrocytes. It functions as a receptor for bone morphogenetic protein (BMP) and induces Indian hedgehog in chondrocytes during skeletal development. Mutations in ACVR1 gene are associated with fibrodysplasia ossificans progressive (PMID: 16642017).

Protocols

Product Specific Protocols
WB protocol for ACVR1 antibody 67417-1-IgDownload protocol
IHC protocol for ACVR1 antibody 67417-1-IgDownload protocol
IF protocol for ACVR1 antibody 67417-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Mol Med

Circulating exosomes repair endothelial cell damage by delivering miR-193a-5p.

Authors - Chang Cao
View Article