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  • KD/KO Validated

GRK2 Polyclonal antibody

GRK2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 13990-1-AP

Synonyms

ADRBK1, BARK, BARK1, Beta ARK 1, BETA ARK1, GRK2



Tested Applications

Positive WB detected inHL-60 cells, Jurkat cells
Positive IP detected inHL-60 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13990-1-AP targets GRK2 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRK2 fusion protein Ag5071
Full Name adrenergic, beta, receptor kinase 1
Calculated molecular weight 689 aa, 80 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC037963
Gene symbol ADRBK1
Gene ID (NCBI) 156
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRK2 (G-protein coupled receptor kinase 2) is also named as ADRBK1 (Beta-adrenergic receptor kinase 1), BARK, and BARK1. G-protein-coupled receptors (GPCRs) transmit extracellular signals across the cell membrane. GPCR kinases (GRKs) desensitize GPCR signals in the cell membrane. GRK2 translocation from the cytoplasm to the cell membrane in response to extracellular signals is critical to the function of the protein, where GRK phosphorylation is an important component of its translocation to the membrane.(PMID:27412951)

Protocols

Product Specific Protocols
WB protocol for GRK2 antibody 13990-1-APDownload protocol
IHC protocol for GRK2 antibody 13990-1-APDownload protocol
IP protocol for GRK2 antibody 13990-1-APDownload protocol
FC protocol for GRK2 antibody 13990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Biother Radiopharm

si-RNA-Mediated Silencing of ADRBK1 Gene Attenuates Breast Cancer Cell Proliferation.

Authors - Chen Zhang
  • KD Validated
humanWB,IHC

Cancer Chemother Pharmacol

The influence of TNF-α and Ang II on the proliferation, migration and invasion of HepG2 cells by regulating the expression of GRK2.

Authors - Zhou-Wei Xu
  • KD Validated
humanWB

Nat Commun

Phospho-selective mechanisms of arrestin conformations and functions revealed by unnatural amino acid incorporation and (19)F-NMR.

Authors - Fan Yang
  • KD Validated