AHCY Monoclonal antibody

AHCY Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1F9B10

Cat no : 66019-1-Ig

Synonyms

Adenosylhomocysteinase, AdoHcyase, AHCY, S adenosylhomocysteine hydrolase, SAHH



Tested Applications

Positive WB detected inHeLa cells, human testis tissue, L02 cells, MCF-7 cells
Positive IHC detected inhuman testis tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66019-1-Ig is AHCY Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen AHCY fusion protein Ag18036
Full Name S-adenosylhomocysteine hydrolase
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC011606
Gene symbol AHCY
Gene ID (NCBI) 191
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).

Protocols

Product Specific Protocols
WB protocol for AHCY antibody 66019-1-IgDownload protocol
IHC protocol for AHCY antibody 66019-1-IgDownload protocol
IF protocol for AHCY antibody 66019-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols