Tested Applications
| Positive WB detected in | LNCaP cells, HeLa cells, human testis tissue, L02 cells, MCF-7 cells, HepG2 cells |
| Positive IHC detected in | human colon cancer tissue, human placenta tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:2000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
66019-1-Ig targets AHCY in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18036 Product name: Recombinant human AHCY protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 116-432 aa of BC011606 Sequence: QTLYFKDGPLNMILDDGGDLTNLIHTKYPQLLPGIRGISEETTTGVHNLYKMMANGILKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVAVVAGYGDVGKGCAQALRGFGARVIITEIDPINALQAAMEGYEVTTMDEACQEGNIFVTTTGCIDIILGRHFEQMKDDAIVCNIGHFDVEIDVKWLNENAVEKVNIKPQVDRYRLKNGRRIILLAEGRLVNLGCAMGHPSFVMSNSFTNQVMAQIELWTHPDKYPVGVHFLPKKLDEAVAEAHLGKLNVKLTKLTEKQAQYLGMSCDGPFKPDHYRY Predict reactive species |
| Full Name | S-adenosylhomocysteine hydrolase |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC011606 |
| Gene Symbol | AHCY |
| Gene ID (NCBI) | 191 |
| RRID | AB_11044194 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P23526 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AHCY antibody 66019-1-Ig | Download protocol |
| IHC protocol for AHCY antibody 66019-1-Ig | Download protocol |
| WB protocol for AHCY antibody 66019-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
