Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83570-4-RR targets ANAPC10 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag8487 Product name: Recombinant human ANAPC10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-185 aa of BC005217 Sequence: MTTPNKTPPGADPKQLERTGTVREIGSQAVWSLSSCKPGFGVDQLRDDNLETYWQSDGSQPHLVNIQFRRKTTVKTLCIYADYKSDESYTPSKISVRVGNNFHNLQEIRQLELVEPSGWIHVPLTDNHKKPTRTFMIQIAVLANHQNGRDTHMRQIKIYTPVEESSIGKFPRCTTIDFMMYRSIR Predict reactive species |
| Full Name | anaphase promoting complex subunit 10 |
| Calculated Molecular Weight | 185 aa, 21 kDa |
| GenBank Accession Number | BC005217 |
| Gene Symbol | ANAPC10 |
| Gene ID (NCBI) | 10393 |
| RRID | AB_3671183 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UM13 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The anaphase promoting complex/cyclosome (APC/C) is a cell cycle-regulated multimeric E3 ubiquitin ligase assembled from 13 individual subunits. The anaphase promoting complex subunit 10 (APC10) is a core subunit of APC/C that is highly conserved in humans. APC10 genetically and physically interacts with a series of subunits of the APC/C and is necessary for the ubiquitination activity of APC/C by enhancing the affinity of the APC/C for its substrate.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ANAPC10 antibody 83570-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ANAPC10 Recombinant monoclonal antibody (240492E7), catalog number 83570-4-RR, has 1 citation to date. The cited publication appeared in Archives of Oral Biology (2025) and investigated ANAPC10 as a potential diagnostic and prognostic biomarker in oral squamous cell carcinoma. The study utilized the antibody for Western blot (WB) in human and mouse samples, placing the research within the fields of oncology, cell cycle biology, and oral pathology.
| Species | Application | Title |
|---|---|---|
Arch Oral Biol Anaphase promoting complex subunit 10 is a potential diagnostic and prognostic biomarker in oral squamous cell carcinoma |
