ARF1 Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 20226-1-AP

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Freight/Packing

Con: 48 μg/150 μl

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Species specificity:
human, mouse

Positive WB detected in:
HeLa cells, HEK-293 cells, HepG2 cells, mouse brain tissue

Positive IP detected in:
HeLa cells

Positive IHC detected in:
human breast cancer tissue, human brain tissue, human breast cancer tissue, human colon cancer tissue, human liver tissue

Positive IF detected in:
HepG2 cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:10-1:100

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Peptide

Full name:
ADP-ribosylation factor 1

Calculated molecular weight:
21 kDa

Observed molecular weight:
18-21 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
ARF1

Synonyms
ADP ribosylation factor 1, ARF1
Background

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment.(PMID: 7759471, PMID: 16042562). The antibody is specific to ARF1.


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