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CL488-conjugated ASS1 Antibody

ASS1 Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse, rat

Applications

IF, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1D1A11

Cat no : CL488-66036

Synonyms

Argininosuccinate synthase, argininosuccinate synthetase 1, ASS, ASS1, Citrulline aspartate ligase, CTLN1



Tested Applications

Positive IF detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66036-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-66036 is CL488-conjugated ASS1 Fusion Protein expressed in E. coli.

Tested Reactivity human, pig, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASS1 fusion protein Ag9314
Full Name argininosuccinate synthetase 1
Calculated molecular weight 412 aa, 47 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC009243
Gene symbol ASS1
Gene ID (NCBI) 445
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).

Protocols

Product Specific Protocols
IF protocol for CL488-conjugated ASS1 antibody CL488-66036Download protocol
Standard Protocols
Click here to view our Standard Protocols