ATG3 Polyclonal antibody

ATG3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11262-2-AP

Synonyms

APG3, APG3 LIKE, APG3L, ATG3, Autophagy related protein 3, hApg3, PC3 96, Protein PC3 96



Tested Applications

Positive WB detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

11262-2-AP targets ATG3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG3 fusion protein Ag1785
Full Name ATG3 autophagy related 3 homolog (S. cerevisiae)
Calculated molecular weight 36 kDa
Observed molecular weight 36-40 kDa
GenBank accession numberBC024221
Gene symbol ATG3
Gene ID (NCBI) 64422
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATG3 antibody 11262-2-APDownload protocol
IHC protocol for ATG3 antibody 11262-2-APDownload protocol
IF protocol for ATG3 antibody 11262-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Ophthalmol

Autophagy activation and the mechanism of retinal microvascular endothelial cells in hypoxia.

Authors - Rong Li
ratWB

Nutr Metab (Lond)

Resveratrol promotes osteoblastic differentiation in a rat model of postmenopausal osteoporosis by regulating autophagy.

Authors - Wei Wang
humanWB

Mar Drugs

7-Acetylsinumaximol B Induces Apoptosis and Autophagy in Human Gastric Carcinoma Cells through Mitochondria Dysfunction and Activation of the PERK/eIF2α/ATF4/CHOP Signaling Pathway.

Authors - Tsung-Chang Tsai
humanWB

Oncotarget

Upregulation of the lncRNA Meg3 induces autophagy to inhibit tumorigenesis and progression of epithelial ovarian carcinoma by regulating activity of ATG3.

Authors - Yin-Ling Xiu
humanWB

Cell Death Dis

Cytoplasmic fragment of CD147 generated by regulated intramembrane proteolysis contributes to HCC by promoting autophagy.

Authors - Bo Wu